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背景 目前研究的目的是评估血小板浓缩物(PCs)中残留白细胞(WBCs的凋亡以及评估在这种介质中炎性细胞因子的浓度和血小板活性与凋亡的相互关系。研究设计和方法 三种独立的方法被应用于评估9份来自单采的单一供者(SD-PCs)或来自混合的血沉棕黄层(BC-PCs)的PCs存在的WECs凋亡,将所有的PCs分成两部分,其中一部分给以照射。PCs贮存于室温4天,样品每天分别分析凋亡、血小板活性(表面标志和可溶的CD62P)和细胞因子的浓度(IL-1α,IL-1β,IL-6,IL-8,和
Background The purpose of the present study is to assess the presence of white blood cells (WBCs) in platelet concentrates (PCs) and to assess the correlation between the concentration of inflammatory cytokines and platelet activity and apoptosis in such media.Study Design and Methods Three The independent method was applied to assess the apoptosis of WECs present in 9 PCs from apheresis (SD-PCs) or from mixed buffy coat (BC-PCs), dividing all PCs into two parts, The samples were stored at room temperature for 4 days. Samples were analyzed daily for apoptosis, platelet activation (surface marker and soluble CD62P) and cytokine concentrations (IL-1α, IL-1β, IL-6, IL -8, and