论文部分内容阅读
目的:研究脑缺血时3’-甲氧基葛根素对谷氨酸、天门冬氨酸、牛磺酸和γ-氨基丁酸的抑制作用,探讨脑缺血损伤再灌注过程中其对神经损伤的保护机制。方法:制作大鼠大脑中动脉阻塞模型,利用脑微透析技术收集大鼠纹状体细胞外液中的氨基酸类神经递质,并3’-甲氧基葛根素灌胃治疗;HPLC荧光检测用药前后4种氨基酸的含量变化。结果:3’-甲氧基葛根素可降低缺血时脑组织中兴奋性氨基酸Asp,Glu的浓度,同时使抑制性氨基酸Tau,GABA含量明显降低。结论:3’-甲氧基葛根素能降低缺血引起的脑内兴奋性氨基酸的毒性,调节脑内兴奋性氨基酸的含量。因此,该药改善脑内氨基酸的变化状态是改善和保护急性期脑梗死大鼠脑内神经保护作用机制之一。
OBJECTIVE: To study the inhibitory effect of 3’-methoxy puerarin on glutamate, aspartate, taurine and γ-aminobutyric acid during cerebral ischemia, Damage protection mechanism. Methods: The middle cerebral artery occlusion model was made in rats. Amino acid neurotransmitters in the extracellular fluid of rat striatum were collected by brain microdialysis technique and treated with 3’methoxy-puerarin. HPLC fluorescence detection Before and after the four kinds of amino acid content changes. Results: 3’-methoxy puerarin decreased the concentrations of Asp and Glu in excitatory amino acids and decreased the contents of inhibitory amino acids Tau and GABA. CONCLUSION: 3’-methoxy puerarin can reduce the brain toxicity of excitatory amino acids caused by ischemia and regulate the content of excitatory amino acids in the brain. Therefore, the drug to improve the status of amino acid changes in the brain is to improve and protect the brain of rats with acute cerebral infarction neuroprotection mechanism.