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目的 观察金属研磨的混合粉尘对大鼠呼吸系统的损伤作用。方法 大鼠经支气管注入磨工粉尘染尘后饲养 2周 ,采用大鼠支气管肺灌洗技术 ,分析灌洗液中细胞含量、分类、存活率及乳酸脱氢酶 (LDH)、碱性磷酸酶 (ALP)活力的变化。结果 染尘大鼠肺泡灌洗液中细胞含量随磨工粉尘染尘剂量的加大而增大 ,且均高于SiO2 尘组。大鼠支气管肺泡灌洗液中性粒细胞比例 [SiO2 粉尘组为 (33 .83± 4 .54) % ,1 0、2 5、50mg/ml磨工粉尘组分别为 (2 6 .50± 3 .99) %、(36 .0 0± 3 .58) %和 (38.0 0±2 .1 0 ) % ]均较对照组 [(2 .83± 0 .75) % ]明显增加 ;巨噬细胞比例 [SiO2 粉尘组为 (62 .1 7± 4 .54) % ,1 0、2 5、50mg/ml磨工粉尘组分别为 (70 .83± 3 .66) %、(60 .83± 2 .1 4 ) %和 (58.1 7± 2 .48) % ]比对照组[(95 .67± 1 .2 1 ) % ]相应降低 ,差异均有显著性 (P <0 .0 1 )。对照组大鼠肺泡灌洗液细胞存活率达80 %以上 ,与磨工粉尘组、TiO2 组的差异有显著性 (P <0 .0 1 )。各染尘组大鼠肺灌洗上清液中LDH、ALP活力明显增加 ,与对照组的差异有显著性 (P <0 .0 1或P <0 .0 5) ,随磨工粉尘染尘剂量加大 ,LDH、ALP活力逐渐升高。结论 磨工粉尘对大鼠肺细胞有损害作用 ,进一步证实磨工粉尘可能对肺脏有致纤
Objective To observe the injury effect of mixed metal dust on the respiratory system in rats. Methods The rats were bred for milking dust for 2 weeks by bronchial injection. The bronchoalveolar lavage technique was used to analyze the content, classification and survival rate of lavage fluid and the effects of lactate dehydrogenase (LDH), alkaline phosphatase (ALP) changes in vitality. Results The content of alveolar lavage fluid in dyed rats increased with the increase of dusting dose of grinding dust, and were higher than that of SiO2 dust group. The ratio of neutrophils in bronchoalveolar lavage fluid of rats [(33.83 ± 4.54)% for SiO 2 dust group and (20.65,50 mg / (36.0 ± 3.58)% and (38.0 ± 2.01)%, respectively, compared with that in the control group [(2.83 ± 0.75)%]; the percentage of macrophages The proportions of [SiO2 dust group (62.1 ± 4.454)%, 1 0,25,50 mg / ml mill dust group were (70.83 ± 3.66)%, (60.83 ± 2) .1 4% and 58.1 7 ± 2 .48%, respectively, were significantly lower than those in the control group (95.67 ± 1.21%). There were significant differences between the two groups (P <0.01). The survival rate of alveolar lavage fluid in control group was more than 80%, which was significantly different from that of grinding dust group and TiO2 group (P <0.01). The activities of LDH and ALP in the lung lavage supernatant of each dyed group were significantly increased (P <0.01 or P <0.05), and the difference with the control group was significant Dose increase, LDH, ALP activity gradually increased. Conclusion mill workers dust lung cells have a damaging effect, further confirmed that grinding dust may cause lung fibrosis