论文部分内容阅读
昆明郊区有50％以上的菜豆程度不同地感染上了花叶病,而且发病率有上升趋势。一般发病的菜豆减产10％～20％,严重发病的减产50％以上甚至绝收。发病症状主要表现为叶片出现黄色与深绿色相间的花叶,或叶面粗糙、皱缩、起疱斑等,全株呈矮缩及丛缩现象,株型矮小,苗期感染容易造成豆苗枯死,后期感染使结荚少而小。在云南省农科院蔬菜试验地内采集表现花叶的小黄金豆病叶,剪碎,加同等重量的0.02MPBS(pH7.0),磨细,少量汁液用磷钨酸钠负染后,JEM-100CXⅡ型电镜观察,其余汁液作为种毒用于接种。
More than 50% of the beans in the suburbs of Kunming are infected with mosaic disease to varying degrees, and the incidence rate is on the rise. The general incidence of beans reduced by 10% to 20%, a serious incidence of more than 50% reduction even absolute. Symptoms showed mainly yellow leaves and dark green and white leaf mosaic, or leaf rough, shrinkage, blistering, etc., the whole plant was dwarfed and constricted, short stature, seedling infection easily lead to bean sprouts Withered, late infection makes the pods small and small. In the Yunnan Provincial Academy of Agricultural Sciences vegetables were collected to show the performance of mosaic leaves of small golden bean disease, cut, add the same weight of 0.02MPBS (pH7.0), ground, a small amount of juice negative staining with sodium phosphotungstate, JEM -100CX Ⅱ type electron microscope observation, the rest of the juice as a kind of virus for vaccination.