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目的 探讨褪黑素对大鼠免疫性结肠炎的影响及有关机制。方法 应用三硝基苯磺酸和乙醇制备大鼠免疫性结肠炎模型。实验设正常对照组、模型对照组、阳性药物对照组 (5 氨基水杨酸 ,1 0 0mg·kg- 1 )、褪黑素给药组 (2 5 ,5 0 ,1 0 0mg·kg- 1 ) ,每天灌肠给药 1次 ,给药时间从制备模型 1wk后开始至实验结束共 3wk。观察大鼠结肠黏膜损伤指数 (CMDI)、粪便隐血实验 (OB)、髓过氧化物酶 (MPO)含量和黏膜病理组织学 (HS)情况 ,并检测结肠组织丙二醛(MDA)、谷胱甘肽过氧化物酶 (GSHPx)、过氧化氢酶(CAT)、超氧化物歧化酶 (SOD)、血浆和结肠组织一氧化氮(NO)含量。结果 模型组大鼠结肠CMDI、HS、OB程度和MPO水平均比正常组升高 ,褪黑素可改善结肠炎大鼠CM DI和HS ,降低MPO水平和粪便OB程度 ,改善结肠黏膜病理损伤 ,大鼠结肠MDA、血浆和结肠NO含量增加 ,结肠SOD、GSHPx和CAT水平降低 ,MT可降低MDA、NO含量 ,增加GSHPx、SOD和CAT水平。结论 MT对大鼠结肠黏膜损伤具有保护作用
Objective To investigate the effect and mechanism of melatonin on immune colitis in rats. Methods The rat model of immunological colitis was established by using trinitrobenzene sulfonic acid and ethanol. The experimental group was divided into normal control group, model control group, positive drug control group (5 Aminosalicylic acid, 100 mg · kg -1), melatonin administration group (25.0 ± 100 mg · kg -1 ), Enema administration once a day, dosing time from the preparation of model 1wk after the end of the experiment a total of 3wk. The colon mucosal injury index (CMDI), excretion occult blood test (OB), myeloperoxidase (MPO) content and mucosal histopathology were observed. The contents of malondialdehyde (MDA), glutathione (GSHPx), catalase (CAT), superoxide dismutase (SOD) and nitric oxide (NO) contents in plasma and colon tissues. Results The levels of CMDI, HS, OB and MPO in model group were higher than those in normal group. Melatonin could improve CM DI and HS in colitis rats, decrease MPO level and the level of stool OB, and improve the pathological changes of colonic mucosa, The contents of MDA, plasma and colon in rats increased, while the levels of SOD, GSHPx and CAT in the colon decreased. MT decreased the content of MDA and NO and increased the levels of GSHPx, SOD and CAT. Conclusion MT has a protective effect on colonic mucosal injury in rats