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为获得烟草合子胚中的优势表达基因，利用 CAP3程序对来自烟草公共数据库的 EST 序列进行组装，利用MISA程序从组装后的EST中筛选SSR位点，将多态性的 SSR 位点在烟草合子文库中进行扩增并对等位基因进行分析。结果表明：具有多态性的16个SSR标记中，有9个基因能从烟草的合子库中成功扩增得到。该研究为筛选烟草合子胚中优势表达基因提供新的途径。“,”In order to obtain the genes of preponderant expression in zygote cells of tobacco,CAP3 program was used to assemble ESTs from GenBank,and MISA program were used to screen micro-satellites.Then polymorphic microG satellites were amplified in cDNA of tobacco zygotes library.The results showed that sixteen polymorphic SSR mark-ers from tobacco ESTs deposited in a public sequence database were developed.Of these,nine genes could be success-fully amplified in cDNA of tobacco zygotes library.The study would provide new channel to screen genes of prepon-derant expression in tobacco zygotes.