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目的:探讨农村正常婴幼儿肠道微生物菌群分布情况,为从饮食上调节婴幼儿肠道微生物菌群的正常分布提供实验基础,从而减少婴幼儿肠道疾病的发生,增进儿童健康.方法:采用分层随机抽样方法抽取陕西省部分农村0-18mo的婴幼儿55人,用 Eugon Tomato、MRS+antibiotics、NN-agar、Shaedler Neo Vanco 平板培养,测定其肠道内粪双歧杆菌属,粪肠杆菌,粪乳酸菌属,粪拟杆菌属,粪产气荚膜梭菌的数量及年龄分布情况.结果:0-18mo 婴幼儿粪便中的益生菌含量与发达国家的健康婴幼儿相比,低了两个对数值,产气荚膜杆菌升高了两个对数值;乳酸菌和双歧杆菌在各组中含量偏低,尤其在4-6mo 最低.结论:及时调节婴幼儿肠道微生物菌群是防病、治病的关键.
Objective: To investigate the distribution of intestinal microflora in normal infants and young children in rural areas and to provide an experimental basis for regulating the normal distribution of intestinal microbial flora in infants and young children, so as to reduce the occurrence of intestinal diseases in infants and young children and improve their health.Methods: Fifty-five infants and young children aged 0-18 months in rural areas of Shaanxi province were sampled by stratified random sampling. Eugon Tomato, MRS + antibiotics, NN-agar and Shaedler Neo Vanco plates were used to determine the intestinal bifidobacteria, Bacillus, Fecal lactic acid bacteria, Bacteroides fecalum, Clostridium perfringens number and age distribution.Results: 0-18mo baby’s stool probiotic content in healthy children than in developed countries, low Two logarithmic values of Clostridium perfringens increased by two logarithm values; lactic acid bacteria and Bifidobacteria were low in each group, especially in 4-6mo.Conclusion: The timely regulation of intestinal microflora in infants and young children is Disease prevention, treatment of the key.