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以邻氨基苯甲酸和咔唑等为原料,经Skraup、Vilsmeier-Haack和Knoevenagel等反应合成了3-{2-[8-(1H-苯并咪唑-2-基)喹啉-2-基]乙烯基}-9-苯甲基-9H-咔唑(探针L),并通过1HNMR、13CNMR、MS对其结构进行了表征。荧光光谱测试结果表明,探针L对Zn~(2+)的选择性好,抗干扰能力较强,50 s内响应,在pH=5~9内对pH不敏感。加入Zn~(2+)后,探针L的荧光强度与Zn~(2+)浓度(0~60μmol/L)呈现良好的线性关系,相关系数R2=0.998 6,检测限为2.003 1×10-8mol/L。Job’s曲线、荧光滴定实验和ESI-MS谱分析表明,探针L与Zn~(2+)的络合比为1∶1。
The synthesis of 3- {2- [8- (1H-benzimidazol-2-yl) quinolin-2-yl] anthranilic acid and carbazole by Skraup, Vilsmeier-Haack and Knoevenagel reaction, Vinyl} -9-benzyl-9H-carbazole (probe L) and its structure was characterized by 1H NMR, 13CNMR, MS. The results of fluorescence spectroscopy showed that probe L has good selectivity for Zn 2+ and strong anti-interference ability, and responds within 50 s. It is insensitive to pH within pH = 5 ~ 9. After addition of Zn 2+, the fluorescence intensity of probe L showed a good linear relationship with the concentration of Zn 2+ (0 ~ 60μmol / L), with a correlation coefficient of R2 = 0.998 6 and a detection limit of 2.003 1 × 10 -8mol / L. Job’s curves, fluorescence titration experiments and ESI-MS spectra showed that the complex ratio of probe L to Zn 2+ was 1: 1.