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目的探讨慢性乙型肝炎病毒(HBV)感染者血清中前S1抗原(Pre-S1Ag)与HBV-DNA、HBV血清标志物之间的相关性,为临床诊治提供参考。方法采用实时定量聚合酶链反应(Real-Time PCR)方法对血清中HBV-DNA进行检测,同时采用化学发光免疫测定法(CLIA)对HBsAg、Pre-S1Ag和HBeAg进行检测。结果 905例标本中,HBV-DNA阳性率为67.96%(615/905),HBV Pre-S1Ag阳性率为68.51%(620/905),差异无统计学意义(P>0.05);615例HBV-DNA阳性患者中,Pre-S1阳性率80.65%(496/615),显著高于HBV-DNA阴性组的Pre-S1阳性率42.76%(124/290),差异有统计学意义(P<0.01);570例HBeAg阳性组中,HBV Pre-S1阳性率为85.08%(485/570),显著高于HBeAg阴性组的Pre-SAg1阳性率40.30%(135/335),差异有统计学意义(P<0.01)。结论Pre-S1Ag与HBV-DNA阳性相关度高,较HBeAg更敏感,互补性和一致性均较好,可作为判断HBV感染与复制的一项重要检测指标。
Objective To explore the correlation between serum Pre-S1Ag, HBV-DNA and HBV serum markers in patients with chronic hepatitis B virus infection and provide reference for clinical diagnosis and treatment. Methods Serum HBV-DNA was detected by Real-Time PCR. The levels of HBsAg, Pre-S1Ag and HBeAg were detected by chemiluminescence immunoassay (CLIA). Results The positive rate of HBV-DNA was 67.96% (615/905) and the positive rate of HBV Pre-S1Ag was 68.51% (620/905) in 905 cases. The difference was not statistically significant (P> 0.05) The positive rate of Pre-S1 in DNA-positive patients was 80.65% (496/615), significantly higher than that in HBV-DNA negative patients (42.76%, 124/290) (P <0.01) . The positive rate of Pre-S1 in HBV-positive patients was 85.08% (485/570) in 570 HBeAg-positive patients, which was significantly higher than that in HBeAg-negative patients (40.30%, 135/335) <0.01). Conclusions Pre-S1Ag is highly correlated with HBV-DNA, more sensitive than HBeAg, with good complementarity and consistency. It can be used as an important index to detect HBV infection and replication.