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目的建立杜仲叶HPLC指纹图谱分析方法,为杜仲叶的质量评价提供参考。方法色谱柱:YMC-Triact C_(18)(250mm×4.6 mm,5.0μm);流动相为乙腈-0.1%H_3PO_4,梯度洗脱;流速为1.0 ml·min~(-1);检测波长208 nm;柱温30℃,测定17个产地的杜仲叶指纹图谱,采用《中药色谱指纹图谱相似度评价系统(2004A版)》软件和SPSS软件对其进行相似度和系统聚类分析。结果确定了杜仲叶HPLC指纹图谱中的30个共有指纹峰,对17批杜仲叶指纹图谱进行相似度分析,相似度在0.828~0.979之间,聚类分析结果为3大类,第I类(S10)为四川省达州市渠县所产,第II类(S3、S2、S7)分别为江西省南昌市湾里区、江西省赣州市崇义县、湖南省张家界市永定区所产,其余为第III类。结论该研究方法准确、可靠、重现性好,可为杜仲叶药材质量评价提供依据。
OBJECTIVE To establish an HPLC fingerprinting method for Eucommia ulmoides leaf, providing a reference for the quality evaluation of Eucommia ulmoides leaves. Methods The chromatographic column was YMC-Triact C18 (250 mm × 4.6 mm, 5.0 μm). The mobile phase consisted of acetonitrile-0.1% H_3PO_4 with a gradient of 1.0 ml · min -1. The detection wavelength was set at 208 nm ; Column temperature 30 ℃, the determination of 17 origin of Eucommia leaf fingerprint, using “Chinese medicine chromatographic fingerprint similarity evaluation system (2004A version)” software and SPSS software similarity and system cluster analysis. Results The fingerprint of Eucommia ulmoides leaves was identified by 30 common fingerprint peaks. The fingerprint similarity of 17 batches of Eucommia ulmoides leaves was analyzed. The similarities were between 0.828 and 0.979. The results of cluster analysis were 3 categories, S10) was produced in Daxian City, Sichuan Province. Category II (S3, S2, S7) was produced in Wanli District, Nanchang, Jiangxi Province, Chongyi County, Ganzhou City, Jiangxi Province and Yongding District, Zhangjiajie City, Hunan Province respectively. The rest were Category III. Conclusion The method is accurate, reliable and reproducible. It can provide the basis for the quality evaluation of Eucommia ulmoides leaves medicinal materials.