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以籼稻单倍体绿芽无性系为材料，研究秋水仙碱等处理对水稻植株染色体二倍化的影响．结果显示：（1）以500mg／L秋水仙碱溶液振荡处理籼稻单倍体绿芽48h，其二倍化率达60％～75％，处理时间过长或处理浓度过高．虽植株染色体二倍率有所提高，但二倍体植株的得率下降；（2）秋水仙碱在愈伤组织阶段处理，其染色体二倍率远高于非愈伤组织化的绿芽二倍率，高达96％～100％，其实未经处理的绿芽愈伤组织再生植株也能获得较高的加倍率；（3）在培养基中添加多效唑（2．5mg／L）也能增加籼稻植株染色体二倍率，由原来的3．3％提高到6．6％．
The indica haploid green shoot clones were used as materials to study the effects of colchicine treatment on chromosome doubling of rice plants. The results showed that: (1) The haploid green shoots of indica rice were shaken for 48h with 500mg / L colchicine solution, the doubling rate reached 60% ~ 75%, the treatment time was too long or the treatment concentration was too high. Although the chromosome doubling rate increased, but the yield of diploid plants decreased; (2) Colchicine treatment at the callus stage, its chromosome double rate is much higher than non-callus double shoot green shoots, Up to 96% ~ 100%. In fact, the regeneration rate of green shoot callus from untreated plants was also higher. (3) The addition of paclobutrazol (2.5 mg / L) Double rate, from the original 3.3% to 6.6%.