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目的探讨甲醛和苯对小鼠遗传毒性的联合作用。方法将健康昆明种小鼠随机分为阴性对照组、甲醛组、苯组、联合组、阳性对照组,进行小鼠骨髓细胞微核试验及精子畸形试验。结果苯组、甲醛组和甲醛、苯联合组小鼠骨髓细胞微核率分别为(13.5±1.27)‰、(11.9±0.99)‰、(25.5±1.58)‰及精子畸形率分别为(101.2±4.22)‰、(94.8±3.71)‰、(167.2±3.78)‰,均明显高于各自的阴性对照组(P<0.01)。析因分析表明,甲醛和苯联合染毒致小鼠骨髓细胞微核率及精子畸形率交互作用显著(P<0.01)。结论析因分析量效曲线随染毒剂量增大而远离,甲醛及苯联合染毒对小鼠遗传毒性有协同作用。
Objective To investigate the combined effect of formaldehyde and benzene on genotoxicity in mice. Methods Healthy Kunming mice were randomly divided into negative control group, formaldehyde group, benzene group, combination group and positive control group. The mice bone marrow micronucleus test and sperm abnormality test were performed. Results The frequencies of micronuclei in bone marrow cells were (13.5 ± 1.27) ‰, (11.9 ± 0.99) ‰, (25.5 ± 1.58) ‰ and sperm deformity rates in benzene group, formaldehyde group and formaldehyde and benzene combination group were (101.2 ± 4.22) ‰, (94.8 ± 3.71) ‰ and (167.2 ± 3.78) ‰, respectively, were significantly higher than their negative control group (P <0.01). Factorial analysis showed that the interaction between formaldehyde and benzene induced micronucleus rate and sperm deformity rate in mouse bone marrow cells significantly (P <0.01). Conclusions The analysis of the dose-response curve with the increase of dose concentration away from the formaldehyde and benzene combined with genotoxicity in mice synergistic effect.