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为了检测ABA对菊花抗蚜性的影响,以切花菊‘神马’为材料,叶面喷施浓度为0(对照)、0.06和0.1 mmol·L-1脱落酸(abscisic acid,ABA),24 h后接种蚜虫。接种7 d开始统计蚜虫数目,每2 d统计一次,连续统计2周,发现0.06 mmol·L-1 ABA处理下,蚜虫数目明显少于对照和0.1 mmol·L-1 ABA处理,即0.06 mmol·L-1 ABA可以提高菊花的抗蚜性。同时研究了外源ABA以及蚜虫接种后菊花叶片的抗氧化酶SOD、POD、CAT活性、H2O2含量和超氧阴离子产生速率。结果表明:未接种蚜虫时,0.06 mmol·L-1 ABA处理下各生理指标均有升高,而0.1 mmol·L-1 ABA处理与对照无显著差异(P<0.05)。0.06mmol·L-1 ABA预处理后接种蚜虫植株中,SOD、POD和CAT活性表现为先上升后下降再上升的趋势,峰值分别为对照的1.6、2.3和1.9倍,H2O2含量和超氧阴离子产生速率具有类似的变化趋势,峰值时均为对照的2.3倍,与对照相比均差异显著。表明ABA可以通过调节活性氧水平来提高菊花的抗蚜性。
In order to examine the effect of ABA on chrysanthemum resistance to aphids, Chrysanthemum cutaneum was used as material, and foliar spraying concentrations of 0 (control), 0.06 and 0.1 mmol·L-1 abscisic acid (ABA), 24 h after inoculation aphids. The number of aphids was counted on the 7th day after inoculation, and the statistics were made every 2 days. The statistics showed that the number of aphids was 0.06 mmol · L-1 ABA treatment with 0.06 mmol·L-1 ABA, L-1 ABA can improve the aphid resistance of chrysanthemum. At the same time, the activities of antioxidant enzymes SOD, POD and CAT, H2O2 content and superoxide anion production rate of exogenous ABA and aphid inoculation were studied. The results showed that all the physiological indexes of ABA treated with 0.06 mmol·L-1 ABI were increased when the aphids were not inoculated, while ABA treatment with 0.1 mmol·L-1 ABO had no significant difference (P <0.05). The activity of SOD, POD and CAT in the aphids plants pretreated with 0.06 mmol·L-1 ABA showed the first rise, then the decrease and then increase, the peak values were 1.6, 2.3 and 1.9 times of the control respectively. The contents of H2O2 and superoxide anion The rate of emergence had a similar trend, with a peak at 2.3 times that of the control, which was significantly different from the control. This indicated that ABA can improve the resistance of chrysanthemum to aphids by regulating the level of reactive oxygen species.