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目的筛选小鼠黑色素瘤B16细胞无源搭载的最佳条件。方法将小鼠黑色素瘤B16细胞纯化后接种在微载体上 ,按不同细胞浓度、血清浓度、微载体量和温度分组。 3 3d后 ,Giemsa染色 ,光境下观察 ,计算细胞存活率。结果细胞存活率 8%和10 %的两组为最佳条件。利用所选条件 ,将细胞搭载于第 2 0颗返回式卫星 ,空间飞行 18d ,细胞返回后 ,经单克隆化 ,获得 110株单克隆细胞 ,存活率为 1 1%。结论筛选出B16细胞无源搭载的最佳条件 ,实际空间飞行表明细胞在空间中的存活时间和生存率满意。
Objective To screen the optimal conditions of passive implantation of mouse melanoma B16 cells. Methods The mouse melanoma B16 cells were purified and seeded on microcarriers. The cells were divided into groups according to cell concentration, serum concentration, microcarrier amount and temperature. After 3 days, Giemsa stained and observed in the light environment to calculate cell viability. Results The cell survival rate of 8% and 10% of the two groups as the best conditions. Using the selected conditions, the cells were mounted on the 20 返 returning satellites in space flight for 18 days. After the cells returned, they were cloned into monoclonal antibodies to obtain 110 monoclonal cells with a survival rate of 11%. Conclusion The optimal conditions of passive carrying of B16 cells were screened out. The actual space flight showed that the survival time and survival rate of B16 cells were satisfactory.