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目的：调查深圳市罗湖区托幼机构儿童蛲虫感染情况，并分析蛲虫感染危险因素。方法以分层抽样法在罗湖区抽取6所托幼机构的489名儿童进行调查。采用透明胶纸肛拭法检查蛲虫卵；同时对托幼机构卫生环境、儿童卫生习惯及家庭卫生情况等进行问卷调查。结果儿童蛲虫感染率为10.22%（50/489）。单因素分析结果显示，可能与蛲虫感染相关（P＜0.05）的因素有：托幼机构档次、年级、睡房地面材质、床铺类型、被褥管理模式、班级独立厕所、盥洗室水龙头类型、父母文化程度、洗澡频率、清洗屁股频率、饭前洗手、便后洗手。多因素Logistic回归分析显示，高年级、被褥混合叠放、母亲学历低、洗屁股频率低以及班级无独立厕所等5个因素为托幼机构儿童蛲虫感染的危险因素。结论控制托幼机构儿童蛲虫感染率，应从规范托幼机构寝具管理、提高家长对蛲虫病的认识、改善儿童的卫生习惯等方面进行综合防制。“,”Objective To understand the prevalence and risk factors of Enterobius vermicularis among preschool children in kindergartens in Luohu District Shenzhen City. Methods A total of 489 children in 6 kindergartens were selected by the stratified sampling method and investigated for E. vermicularis infection by the cellophane anal swab technique. The information of sanitary condition of the kindergartens personal hygiene and family hygiene were investigated by questionnaire. Results The infection rate of E. vermicularis was 10.2% 50/489 . The single factor analysis indicated that the following factors might re?lated to the infection the different classes of kindergartens grades ground of bed room private toilet types of taps and beds bed management education levels of parents frequency of shower and washing anus and washing hands before meal and after WC. The multivariate Logistic analysis indicated that the bed management education level of mothers frequency of washing anus and private toilet were independent risk factors for E. vermicularis infection. Conclusion To control the infection of E. vermicularis the circumstance and management of kindergartens parents knowledge of E. vermicularis infection and chil?dren s healthy habit need improve.