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目的建立一个控制性、重复性好,并可分级的骨骼肌挫伤动物模型。方法采用自行设计的机械式撞击装置,以撞击速度(velocity,v)和形变(deformation,DF)作为外力参数,并保持较恒定的撞击时间,使击锤打击瞬间速度不同,造成轻、中、重不同程度的大鼠腓肠肌挫伤。结果在肉眼、HE染色观察下,39只大鼠腓肠肌出现可分级的病理学改变。在低撞击水平(v:2m/s,DF:5.5mm),轻度挫伤组病理改变局限于大鼠腓肠肌肌外膜和腓肠肌表层部位;在中度撞击水平(v:2.5m/s,DF:6.5mm),中度挫伤组病理改变可见于大鼠腓肠肌肌外膜和大部分腓肠肌;在高撞击水平(v:3m/s,DF:7.5mm),重度挫伤组病理改变除中度挫伤组累及部位外,还伤及比目鱼肌。结论该模型制作出常见的钝力性骨骼肌挫伤,以撞击速度和形变作为外力参数,成功建立了机械骨骼肌挫伤分级模型,致伤程度较一致,是骨骼肌挫伤研究较合适的动物模型,为深入研究钝力性骨骼肌挫伤奠定了基础。
Objective To establish a controllable, repetitive and scalable animal model of skeletal muscle contusion. Methods The self-designed mechanical impact device was used as the external force parameters to set the impact velocity (velocity) and deformation (DF) as the external force parameters and to maintain a relatively constant impact time so that the instantaneous impact velocity of the hammer would be different, Severe degrees of rat gastrocnemius contusion. Results Under the naked eye and HE staining, graded pathological changes of gastrocnemius were observed in 39 rats. At the low impact level (v: 2m / s, DF: 5.5mm), the pathological changes in mild contusion group were confined to the superficial part of the gastrocnemius and gastrocnemius in rats. At moderate impact level (v: 2.5m / s, DF : 6.5mm). The pathological changes in moderate contusion group were found in the gastrocnemius and the gastrocnemius of the gastrocnemius rats. At high impact level (v: 3m / s, DF: 7.5mm) Group involved parts, but also injured soleus. Conclusions The blunt force-induced skeletal muscle contusion is made by this model. Based on the impact velocity and deformation as the external force parameters, the model of mechanical skeletal muscle contusion is successfully established. The injury degree is more consistent. It is a suitable animal model for the study of skeletal muscle contusion. Which laid the foundation for the further study of blunt force skeletal muscle contusion.