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目的:探讨藏药多血康对缺氧大鼠红细胞增多症的预防作用。方法:雄性大鼠在5 000m高原环境模拟舱中缺氧(22h/d),每2天1次腹腔注射CoCl2溶液(5.5 mg/kg)以建立红细胞增多症模型。经颈动脉插管取血,然后测定大鼠红细胞数、血红蛋白和红细胞压积,并检测脑组织HIF-1α、血清EPO、肾脏EPO mRNA等,观察多血康对HAPC治疗作用。结果:红细胞增多症模型组大鼠的红细胞数、血红蛋白和红细胞压积增加,血清EPO含量增加,HIF-1α、EPO表达增加;与高原模型组相比,多血康高、低剂量(0.16g/kg、0.04g/kg)组大鼠的红细胞数、血红蛋白和红细胞压积值均明显下降,且高剂量组较低剂量组显著,但仍高于平原组;同时多血康可以降低缺氧大鼠血清EPO含量、下调EPO、HIF-1α表达。结论:多血康对高原红细胞增多症有一定防治作用。
Objective: To explore the preventive effect of Tibetan medicine Duoxuekang on hypoxemia in rats with polycythemia. Methods: Male rats were hypoxic (22 h / d) in a simulated altitude of 5 000 m plateau, and intraperitoneal injection of CoCl2 solution (5.5 mg / kg) every 2 days to establish the polycythemia model. Blood samples were taken from the carotid artery. The number of erythrocytes, hemoglobin and hematocrit of the rats were measured. HIF-1α, EPO and EPO mRNA in the brain tissue were detected. The therapeutic effect of Doxinkang on HAPC was observed. Results: The number of erythrocyte, hemoglobin and hematocrit increased in rats with polycythemia, the levels of serum EPO increased and the expressions of HIF-1α and EPO increased. Compared with the model group, / kg, 0.04g / kg), the hemoglobin and hematocrit values of the rats in the high-dose group were significantly lower than those in the low-dose group, but were still higher than those in the plain group; meanwhile, Duoxikang could reduce the hypoxia The level of serum EPO in rats decreased EPO and HIF-1α expression. Conclusion: Dokangkang can prevent and treat plateau polycythemia.