论文部分内容阅读
本文采用RACE技术,从巴西橡胶树中克隆到一个小热激蛋白基因。该基因全长cDNA为1 002 bp,最长开放阅读框645 bp,预测编码蛋白包含214个氨基酸残基,与植物sHSP23.8蛋白具高度相似,将该基因命名为HbsHSP23.8。生物信息学分析显示,HbsHSP23.8具有α-晶体蛋白保守结构域,可能定位在叶绿体,HbsHSP23.8蛋白中预测到19个磷酸化位点。二级结构预测结果显示HbsHSP23.8由α螺旋、β转角、延伸链和随机卷曲组成。实时定量RT-PCR分析结果表明,HbsHSP23.8在巴西橡胶树胶乳、叶片、树皮、雄花、雌花、花药中均有表达。在橡胶树叶片不同发育时期,HbsHSP23.8表达存在明显变化。此外,HbsHSP23.8表达受高盐、干旱、低温、乙烯和茉莉酸处理调控,表明HbsHSP23.8可能在巴西橡胶树逆境胁迫应答、乙烯和茉莉酸信号途径中发挥作用。
In this paper, we used RACE technology to clone a small heat shock protein gene from the rubber tree in Brazil. The full-length cDNA of this gene was 1 002 bp, the longest open reading frame was 645 bp, and the predicted protein contained 214 amino acid residues, which was highly similar to the plant sHSP23.8 protein. The gene was named as HbsHSP23.8. Bioinformatics analysis showed that HbsHSP23.8 has a-crystallin conserved domain, which may be located in the chloroplast, HbsHSP23.8 protein predicted 19 phosphorylation sites. Secondary structure prediction results show that HbsHSP23.8 consists of α-helix, β-turn, extended chain and random curl. Real-time quantitative RT-PCR analysis showed that HbsHSP23.8 was expressed in rubber latex, leaves, bark, male flower, female flower and anther. In different developmental stages of rubber tree leaves, HbsHSP23.8 expression has obvious changes. In addition, HbsHSP23.8 expression was regulated by high salt, drought, low temperature, ethylene and jasmonic acid treatment, indicating that HbsHSP23.8 may play an important role in the response to stress of Acacia confusa and ethylene and jasmonic acid signaling pathways.