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目的制备小鼠肾炎模型并观察双氢青蒿素(dihydroartemisinine,DHA)对模型小鼠细胞因子肿瘤坏死因子-α(tunor necrosis factor,TNF-α)和白细胞介素-6(inter leukin-6,IL-6)的影响以及小鼠肾脏的病理变化。方法取雄性昆明种小鼠120只,随机分为正常对照组、脂多糖(lipopolysaccharides,LPS)组、LPS+肾匀浆组及DHA治疗组;分别于12、24、48h取血,酶联免疫吸附试验检测血清中TNF-α和IL-6的含量,苏木精-伊红染色法观察小鼠肾脏的病理变化。结果造模48hLPS+肾匀浆组小鼠肾小球出现炎性细胞浸润,而正常对照组未见异常;LPS组及DHA治疗组仅有轻微的病理改变。LPS刺激使小鼠血清TNF-α和IL-6含量高于正常水平(P<0.01),但有随时间不断下降的趋势;LPS+肾匀浆组较正常对照组TNF-α和IL-6含量升高(P<0.01);DHA可显著下调模型小鼠血清TNF-α的水平(P<0.01),但对IL-6的影响相对较小(P>0.05)。结论运用改良的造模方法LPS+肾匀浆建立肾炎模型效果良好;DHA可以调节模型小鼠炎症因子TNF-α和IL-6的释放,具有一定的改善模型小鼠肾炎症状的作用。
Objective To investigate the effects of dihydroartemisinine (DHA) on tumor necrosis factor (TNF) -α and interleukin-6 (IL-6) IL-6) and the pathological changes in mouse kidneys. Methods Totally 120 male Kunming mice were randomly divided into normal control group, lipopolysaccharides (LPS) group, LPS + kidney homogenate group and DHA treatment group. Blood was collected at 12, 24 and 48 hours respectively, and enzyme linked immunosorbent assay The contents of TNF-α and IL-6 in serum were detected by the test, and the pathological changes in the kidney of mice were observed by hematoxylin-eosin staining. Results The inflammatory cell infiltration was found in the glomeruli in the 48hLPS + kidney homogenate group, but no abnormality was found in the normal control group. The LPS group and the DHA treatment group had only slight pathological changes. The levels of TNF-α and IL-6 in serum of mice in LPS plus kidney group were higher than those in normal group (P <0.01), but decreased with time. LPS + (P <0.01). DHA could significantly decrease the level of serum TNF-α in model mice (P <0.01), but had less effect on IL-6 (P> 0.05). Conclusion DPS can improve the model of nephritis by using modified model making method. DHA can regulate the release of inflammatory cytokines TNF-α and IL-6, which can improve the nephritis symptoms of model mice.