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目的:研究小檗碱对脂肪细胞增殖、糖代谢及地塞米松诱导3T3-L1脂肪细胞中葡萄糖转运蛋白4(GLUT4)mRNA表达的影响。方法:用四甲基偶氮唑盐(MTT)方法检测3T3-L1前脂肪细胞及脂肪细胞的增殖情况,检测小檗碱对3T3-L1前脂肪细胞及脂肪细胞培养基中葡萄糖浓度的影响;采用地塞米松诱导胰岛素抵抗细胞模型,分别给予罗格列酮、小檗碱进行干预,用RT-PCR检测GLUT4 mRNA的表达。结果:在DMEM高糖培养基中,小檗碱可促进3T3-L1前脂肪细胞增殖,抑制3T3-L1脂肪细胞增殖,作用呈明显量效关系;使3T3-L1前脂肪细胞及3T3-L1脂肪细胞葡萄糖消耗量增加,呈明显量效关系,同时小檗碱组GLUT4 mRNA的表达升高。结论:小檗碱可以显著促进前脂肪细胞的增殖,抑制脂肪细胞的增殖;同时具有显著的降糖作用。且小檗碱能上调脂肪细胞GLUT4 mRNA的表达,这提示小檗碱改善胰岛素抵抗的作用机制可能与罗格列酮不同。
Objective: To investigate the effects of berberine on the proliferation of adipocytes, glucose metabolism and glucose transporter 4 (GLUT4) mRNA expression induced by dexamethasone in 3T3-L1 adipocytes. Methods: The proliferation of 3T3-L1 preadipocytes and adipocytes was detected by MTT assay, and the effect of berberine on glucose concentration in 3T3-L1 preadipocytes and adipocyte medium was detected. The model of insulin resistance was induced by dexamethasone, and rosiglitazone and berberine were respectively administered to interfere the expression of GLUT4 mRNA by RT-PCR. Results: In DMEM high glucose medium, berberine could promote the proliferation of 3T3-L1 preadipocytes and inhibit the proliferation of 3T3-L1 adipocytes. The effect of 3T3-L1 preadipocytes and 3T3-L1 fat Cell glucose consumption increased, showing a significant dose-effect relationship, while berberine group GLUT4 mRNA expression increased. Conclusion: Berberine can significantly promote the proliferation of preadipocytes, inhibit the proliferation of adipocytes, and have a significant hypoglycemic effect. And berberine can up-regulate the expression of GLUT4 mRNA in adipocytes, suggesting that the mechanism of berberine to improve insulin resistance may be different from rosiglitazone.