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探讨ｃ－ｍｙｃ基因表达在雏鸡基底乳头（ｂａｓｉｌａｒ ｐａｐｉｌｌａ， ＢＰ）庆大霉素损伤后听毛细胞再生过程中的意义。方法：应用原位杂交和免疫组化技术检测庆大霉素停药后第 １，３，７，１４，２１和 ２８ ｄ时ＢＰ损伤区域ｃ－ｍｙｃ ｍＲＮＡ和 ｃ－ｍｙｃ蛋白表达情况。结果：ＢＰ修复过程中ｃ－ｍｙｃ ｍＲＮＡ和 ｃ－ｍｙｃ蛋白表达水平明显升高，修复 ７ｄ时表达水平达高峰，修复 １４ ｄ后ＢＰ中ｃ－ｍｙｃ基因表达水平开始下降。结论：提示ｃ－ｍｙｃ基因表达是毛细胞再生过程中的一个重要分子事件，可能与前体细胞增殖有关。
To investigate the significance of c-myc gene expression in hair cell regeneration after gentamicin injury in basilar papilla (BP). Methods: The expressions of c-myc mRNA and c-myc protein in BP-injured area were detected by in situ hybridization and immunohistochemistry at 1, 3, 7, 14, 21 and 28 days after gentamicin treatment. Results: The expression of c-myc mRNA and c-myc protein in BP repair group was significantly higher than that in BP repair group. The expression level of c-myc mRNA reached the peak at the 7th day after repair, and the expression of c-myc gene began to decrease after 14 days of repair. Conclusion: It is suggested that c-myc gene expression is an important molecular event in hair cell regeneration and may be related to the proliferation of precursor cells.