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目的:初步分析人源性多肽pCM1-9的抗菌作用机制。方法:利用荧光素掺入破损细胞膜的原理,采用流式荧光激活细胞分析(FACScan)和扫描电子显微镜分析pCM1-9对靶菌膜的作用机制。结果:FACS分析法结果显示pCM1-9处理后的大肠杆菌PI着染细菌比例明显高于未处理组,并且随作用时间的延长,PI着染BL-21菌比例增大,20 m in时达最高水平,20 m in以后反而降低;扫描电子显微镜观察到pCM1-9处理后的大肠杆菌有明显的表面形态学的改变,如细菌体积缩小、细菌表面出现皱缩、凹陷似有孔洞形成。结论:人源性多肽pCM1-9可能作用于细菌细胞壁和(或)细胞膜,通过改变其通透性而发挥杀菌作用。
OBJECTIVE: To preliminary analyze the antibacterial mechanism of human polypeptide pCM1-9. Methods: The mechanism of pCM1-9 on target bacteria membrane was analyzed by fluorescence activated cell assay (FACScan) and scanning electron microscopy (FACScan). Results: The results of FACS analysis showed that the proportion of PI-infected bacteria in E.coli PI treated with pCM1-9 was significantly higher than that in untreated group, and the percentage of PI-infected BL-21 increased with the prolongation of treatment time. The highest level decreased after 20 m in. Scanning electron microscopy showed that there were obvious morphological changes of E. coli after treatment with pCM1-9. For example, when the volume of bacteria was reduced, the surface of bacteria appeared to shrink and the depression appeared to have holes. CONCLUSION: The human polypeptide pCM1-9 may act on the bacterial cell wall and / or cell membrane and exert its bactericidal effect by changing its permeability.