论文部分内容阅读
目的:制备免疫纳米荧光微球(Immunologic Nano-Fluorescence beads,INFB)并对其粒径和功能进行了研究。方法:应用纳米生物技术、免疫学技术和现代细胞生物学研究技术进行研究。选用K562细胞和Hela细胞为靶细胞,经INFB作用48h后,MTT法检测各组OD值。选用苏木、大血藤、秦巴西菇水提液和茶多糖诱导脐血单个核细胞,用INFB标记,FACS检测结果。结果:扫描电镜显示,荧光微球粒径平均为136.9nm。MTT结果显示,各实验组数据与对照组比较,无统计学意义(p>0.5),表明INFB对所选靶细胞无生物毒性作用。中药有效成分诱导人脐血单个核细胞后,CD34、CD33、CD14、CD119、CD19、CD4阳性细胞的数值,呈现剂量依赖关系,经统计学分析,药物浓度与检测的靶细胞数量呈现良好的线性关系(r=0.745376～0.986402)。结论:INFB可用于医学检验学、免疫学标记和中药活性成分的靶向作用研究等领域。
Objective: To prepare Immunological Nano-Fluorescence beads (INFB) and study its particle size and function. Methods: Nanobiotechnology, immunological techniques, and modern cell biology research techniques were used for research. K562 cells and Hela cells were used as target cells. After being treated with INFB for 48 h, the OD value of each group was detected by MTT assay. Mononuclear cells of umbilical cord blood were induced by using hematoxylin, hematochezia, aqueous extracts of Pleurotus citrinopileatus and tea polysaccharide, and labeled with INFB, and the results were detected by FACS. RESULTS: Scanning electron microscopy showed that the average particle size of fluorescent microspheres was 136.9 nm. The MTT results showed that there was no statistically significant difference between the experimental data and the control group (p>0.5), indicating that INFB had no biological toxicity to the selected target cells. The value of CD34, CD33, CD14, CD119, CD19, and CD4 positive cells in a dose-dependent manner after induction of human umbilical cord blood mononuclear cells by the active ingredients of traditional Chinese medicine showed statistically significant correlation between the concentration of the drug and the number of target cells detected. Relationship (r=0.745376 to 0.986402). CONCLUSIONS: INFB can be used in medical tests, immunological markers, and targeted studies of active ingredients of traditional Chinese medicines.