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目的观察激素对哮喘小鼠血清干细胞因子(SCF)的影响。方法24只雄性BALB/c小鼠随机分成正常对照组(A组)、哮喘模型组(B组)、地塞米松干预哮喘组(C组),每组8只。用HE染色观察气道病理改变,ELISA法检测小鼠血清中SCF含量。结果血清SCF含量:B组(114.9±27.3)pmol/L,明显高于A组的(48.6±11.2)pmol/L(P<0.01)或C组的(53.0±7.0)pmol/L(P<0.01)。B组肺组织中嗜酸性粒细胞(EOS)和淋巴细胞总数为(32.6±4.5)个/mm2,明显高于A组的(6.6±1.2)个/mm2(P<0.01)或C组的(11.4±1.9)个/mm2(P<0.05);B组管壁面积/支气管管腔内周长(WA/Pi)和支气管平滑肌面积/支气管管腔内周长(ASM/Pi)分别为(1.196±0.111)mm和(0.292±0.027)mm,明显大于A组的(0.571±0.057)mm和(0.139±0.014)mm(P<0.01)或C组的(0.857±0.119)mm和(0.175±0.024)mm(P<0.05)。结论SCF在哮喘的发病过程中起作用;地塞米松可能通过抑制血清SCF表达抑制哮喘小鼠气道炎症。
Objective To observe the effects of hormones on serum levels of stem cell factor (SCF) in asthmatic mice. Methods Twenty - four male BALB / c mice were randomly divided into normal control group (group A), asthmatic model group (group B) and dexamethasone intervention group (group C), with 8 mice in each group. The pathological changes of the airway were observed by HE staining, and the level of SCF in the serum of mice was detected by ELISA. Results Serum SCF levels were significantly higher in group B (114.9 ± 27.3) pmol / L than those in group A (48.6 ± 11.2) pmol / L or (53.0 ± 7.0) pmol / L in group C (P < 0.01). The total number of eosinophils (EOS) and lymphocytes in group B was (32.6 ± 4.5) / mm2, which was significantly higher than that of group A (6.6 ± 1.2) / mm2 (P <0.01) or group C 11.4 ± 1.9) /mm2 (P <0.05). The area of wall of group B / WA / Pi and ASM / Pi of bronchus were (1.196 ± 0.111 mm and 0.292 ± 0.027 mm respectively, which were significantly higher than those in group A (0.571 ± 0.057) mm and (0.139 ± 0.014) mm (0.857 ± 0.119) mm and (0.175 ± 0.024) mm ) mm (P <0.05). Conclusions SCF plays a role in the pathogenesis of asthma. Dexamethasone may inhibit airway inflammation in asthmatic mice by inhibiting the expression of SCF in serum.