论文部分内容阅读
目的 观察解放军小汤山 SARS传染病医院一线医护人员与 SARS患者密切接触后免疫功能受到影响的情况。方法 应用 EL ISA方法 ,对随机抽取的 397名解放军小汤山 SARS传染病医院一线医护人员血清标本 ,进行抗 SARS- Co V抗体 Ig G、Ig M阴阳性测定 ,并对其中 98人应用流式细胞测定技术分析淋巴细胞亚群。结果抗 SARS抗体 Ig G阳性 2例 ,阳性率 0 .5 % ,Ig M全部阴性 ;NK细胞、B细胞、CD4 + 、CD8+ 、CD3+ 及 CD4 + / CD8+ 比值与正常人参考值 ,差异无显著性 (P>0 .0 5 )。结论 与 SARS患者的密切接触对一线医护人员的免疫功能无负面影响 ,并表明解放军小汤山医院对医护人员所采取的防护措施是非常有效的。
Objective To observe the impact of immune function on the frontline medical staff of the Xiaotangshan SARS Infectious Diseases Hospital of People’s Liberation Army (PLA) in close contact with SARS patients. Methods Serum samples of 397 PLA medical staff from Xiaotangshan SARS Infectious Diseases Hospital were randomly selected for ELISA. The anti-SARS-Co V antibodies Ig G, Ig M were detected. 98 of them were treated with flow cytometry Assay technique to analyze lymphocyte subsets. Results IgG positive anti-SARS antibodies in 2 cases, the positive rate of 0.5%, IgM all negative; NK cells, B cells, CD4 +, CD8 +, CD3 + and CD4 + / CD8 + ratio and the normal reference value, no significant difference (P> 0.05). Conclusions Close contact with patients with SARS has no negative impact on the immune function of frontline medical staff and shows that the Xiaotangshan Hospital of People’s Liberation Army is very effective for the protective measures taken by medical staff.