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目的:观察三七总皂苷主要成分人参皂苷Rg1和Rb1在心肌缺血复合肿瘤病理环境下的作用,并通过研究血管新生探索其可能的作用机制。方法:采用小鼠经皮下移植LLCs肿瘤细胞及腹腔注射异丙肾上腺素的方法制备肿瘤复合心肌缺血动物模型,再随机分为模型组、人参皂苷Rg1组、人参皂苷Rb1组;另设正常对照组。Rg1组和Rb1组分别予腹腔注射Rg1、Rb1(50 mg/kg);正常组与模型组给予等体积生理盐水。连续干预15 d后,比较各组小鼠的肿瘤生长情况、心肌组织病理形态学变化及通过免疫组织化学的方法检测心肌和肿瘤组织中CD34、vWF的蛋白表达。结果:人参皂苷Rg1及Rb1治疗组小鼠移植肿瘤生长显著受到抑制,其平均瘤重显著低于模型组(P<0.05);两组心肌组织缺血损伤显著改善,胶原含量明显减少(P<0.05,P<0.01);同时心肌组织中CD34和vWF蛋白表达量显著升高,而肿瘤组织中CD34和vWF蛋白表达量显著降低,与模型组比较差异有统计学意义(P<0.05,P<0.01)。结论:三七总皂苷主要活性成分人参皂苷Rg1和Rb1在心肌缺血复合肿瘤病理环境下显著抑制LLCs肿瘤生长,并显著改善心肌缺血损伤,其双向作用的机制可能与对血管生成双向调节作用有关。
OBJECTIVE: To observe the role of ginsenosides Rg1 and Rb1, the main constituents of Panax notoginseng saponins, in the pathological environment of myocardial ischemia complicated with cancer and explore its possible mechanism by studying angiogenesis. Methods: The animal models of tumor combined myocardial ischemia were established by subcutaneous transplantation of LLCs tumor cells and isoprenaline by intraperitoneal injection in mice. The rats were randomly divided into model group, ginsenoside Rg1 group and ginsenoside Rb1 group. group. Rg1 and Rb1 were intraperitoneally injected with Rg1 and Rb1 (50 mg / kg) respectively. The normal and model groups were given equal volume of normal saline. After 15 days of continuous intervention, tumor growth, myocardial histopathological changes and protein expression of CD34 and vWF in myocardium and tumor tissue were detected by immunohistochemistry. Results: The tumor growth of mice treated with ginsenoside Rg1 and Rb1 was significantly inhibited, and the mean tumor weight was significantly lower than that of the model group (P <0.05). The ischemic injury of myocardium in both groups was significantly improved and the collagen content was significantly decreased (P < 0.05, P <0.01). At the same time, the expressions of CD34 and vWF in myocardium were significantly increased, while the expressions of CD34 and vWF in tumor tissue were significantly lower than those in model group (P <0.05, P < 0.01). CONCLUSION: Ginsenoside Rg1 and Rb1, the major active components of Panax notoginseng saponins, significantly inhibit the growth of LLCs and improve myocardial ischemic injury significantly in the pathological setting of myocardial ischemic complex. The mechanism of bidirectional effect may be related to the regulation of angiogenesis related.