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应用非甾体抗炎药消炎痛(20mg/kg)抑制hCG 诱发的家兔排卵,注射后的6、9、12、72小时剖腹观察排卵数,并取材进行透射电镜观察和酶组织化学观察。甾体激素合成酶类的组织化学和颗粒细胞内某些细胞器的电镜立体定量学测定结果表明:消炎痛抑制了排卵,却不影响颗粒细胞向黄体细胞的转变。透射电镜观察表明:在排卵前,实验组和对照组最明显的不同是:实验组的外膜和白膜很少见到胶原溶解现象,且表面上皮细胞内含的致密小体不释放其内容物。结果提示:消炎痛主要作用于排卵前卵泡顶部的成纤维细胞和表面上皮细胞,这可能与其抑制了某些与排卵有关的酶的释放有关。
Indomethacin (20mg / kg), a non-steroidal anti-inflammatory drug, was used to inhibit ovulation in rabbits induced by hCG. The number of ovulation was observed by laparotomy 6, 9, 12, and 72 hours after injection. The cells were observed by transmission electron microscopy and enzyme histochemistry. Histochemistry of steroid hormone synthetases and electron microscopic quantitative determination of some organelles in granulosa cells showed that indomethacin inhibited ovulation without affecting the transition from granulosa cells to luteal cells. Transmission electron microscopy showed that the most obvious difference between the experimental group and the control group before ovulation was that the dissolution of collagen in the outer membrane and the albuginea membrane of the experimental group seldom seen and the dense bodies contained in the surface epithelial cells did not release their contents Things. The results suggest that indomethacin mainly acts on fibroblasts and surface epithelial cells on the top of ovulation follicles, which may be related to the inhibition of some ovulation-related enzymes.