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目的探讨渗透压法检测冰冻解冻去甘油红细胞甘油残留量的可行性。方法用生理盐水、氯化钠-腺嘌呤-葡萄糖-甘露醇-磷酸二氢钠(MAP)液、血浆等不同介质溶液制备成含不同甘油浓度的洗涤红细胞,以模拟冰冻解冻去甘油红细胞,用渗透压仪测定其渗透压值,进行线性回归处理与相关系数的计算;并进行溶血干扰试验及重复性试验。结果渗透压值与甘油含量之间有很好的线性关系,但相同浓度的甘油在不同介质溶液(生理盐水、MAP液、血浆)悬浮的洗涤红细胞中的渗透压值有差异,生理盐水、MAP液、血浆悬浮的甘油浓度为10 g/L的洗涤红细胞的渗透压值分别为434、467、461 mmol/kg。游离血红蛋白在1.466~7.713 g/L时,渗透压测定值的差异无统计学意义。结论渗透压与甘油含量之间有良好的线性关系,溶血对渗透压测定影响小,可用于冰冻解冻去甘油红细胞的甘油残留量检测,且操作简单、快捷,但测定时应考虑制品的悬浮介质。
Objective To investigate the feasibility of using osmometry to detect glycerol residues in frozen thawed glycerol red blood cells. Methods The washed red blood cells with different concentrations of glycerol were prepared by using different saline solution, sodium chloride-adenine-dextrose-mannitol-sodium dihydrogen phosphate (MAP) solution and plasma in order to simulate the freezing and thawing of glycerol red blood cells The osmotic pressure was used to measure the osmotic pressure, the linear regression was processed and the correlation coefficient was calculated. The hemolysis interference test and the repeatability test were carried out. Results There was a good linear relationship between osmotic pressure and glycerol content. However, osmotic pressure of the same concentration of glycerol in washed red blood cells suspended in different media solutions (saline, MAP solution, plasma) Osmotic pressure of washed red blood cells with liquid and plasma suspended glycerol concentration of 10 g / L were 434, 467 and 461 mmol / kg, respectively. When the free hemoglobin was between 1.466 and 7.713 g / L, the difference of osmotic pressure was not statistically significant. Conclusion There is a good linear relationship between osmotic pressure and glycerol content. Hemolysis has little effect on the determination of osmotic pressure. It can be used for the determination of glycerol residues in frozen-thawed glycerol red blood cells. The operation is simple and quick. However, the suspension medium .