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通过分散聚合法制备微米级单分散聚甲基丙烯酸甲酯(PMMA)微球,以此为空白载体,交替包覆聚乙烯亚胺(PEI)和修饰有柠檬酸钠的Fe3O4纳米粒子,得到了磁性微球(PMMA-M)。通过偶联反应分别成功制备了罗丹明B(RhB)、羧基荧光素(CFRC)与氨丙基三乙氧基硅烷(APTES)的荧光硅烷化试剂,采用Stober法将荧光硅层成功包覆在磁球表面。最终制备了单分散、超顺磁的荧光核/壳/壳型聚合物微球(PMMA-M-F)。通过透射电子显微镜观察微球形貌圆整,振动样品磁强计表明微球的饱和磁化强度高达9.8emu/g,并且具有超顺磁性。激光共聚焦显微镜显示多功能微球具有荧光。
Micro-scale monodispersed PMMA microspheres were prepared by dispersion polymerization. As blank carriers, PEI and sodium citrate modified Fe3O4 nanoparticles were alternately coated to obtain Magnetic microspheres (PMMA-M). Fluorescent silanization reagents of rhodamine B (RhB), carboxyfluorescein (CFRC) and aminopropyltriethoxysilane (APTES) were successfully prepared by the coupling reaction. The stained silica layer was successfully coated by Stober method Magnetic ball surface. Finally, monodisperse and superparamagnetic fluorescent core / shell / shell polymer microspheres (PMMA-M-F) were prepared. The morphology of the microspheres was observed by transmission electron microscopy. The vibrating sample magnetometer showed that the saturation magnetization of microspheres was as high as 9.8emu / g and superparamagnetism. Laser confocal microscopy showed multi-functional microspheres have fluorescence.