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The purpose of this study was to characterize Ta14 S homoeologs and assess their functions in wheat seed development.The genomic and c DNA sequences of three Ta14 S homoeologous genes encoding 14-3-3 proteins were isolated.Sequence analysis revealed that the three homoeologs consisted of five exons and four introns and were very highly conserved in the coding regions and in exon/intron structure,whereas the c DNA sequences were variable in the 5′ and 3′-UTR.The three genes,designated as Ta14S-2A,Ta14S-2B and Ta14S-2D,were located in homoeologous group 2 chromosomes.The polypeptide chains of the three Ta14 S genes were highly similar.These genes were most homologous to Hv14 A from barley.Real-time quantitative PCR indicated that the three Ta14 S genes were differentially expressed in different organs at different developmental stages and all exhibited greater expression in primary roots of 1-day-old germlings than in other tissues.Comparison of the expression patterns of the three homoeologous genes at different times after pollination also revealed that their expression was developmentally regulated.The transcription of Ta14S-2B was clearly higher during seed germination,whereas expressions of Ta14S-2A and Ta14S-2D were up-regulated at the beginning of seed imbibition(0–12 h),but declined thereafter.The results suggested that the three Ta14 S homoeologous genes have regulatory roles in seed development and germination.
The purpose of this study was to characterize Ta14 S homoeologs and assess their functions in wheat seed development. The genomic and c DNA sequences of three Ta14 S homoeologous genes encoding 14-3-3 proteins were isolated. Sequence analysis revealed that the three homoeologs consisted of five exons and four introns and were very highly conserved in the coding regions and in exon / intron structure, whereas the c DNA sequences were variable in the 5 ’and 3’-UTR.The three genes, designated as Ta14S-2A, Ta14S -2B and Ta14S-2D, were located in homoeologous group 2 chromosomes. The polypeptide chains of the three Ta14 S genes were more similar. These genes were most homologous to Hv14 A from barley. Real-time quantitative PCR indicated that the three Ta14S genes were differentially expressed in different organs at different developmental stages and all shows greater expression in primary roots of 1-day-old germlings than in other tissues. Comparison of the expression patterns of the three h omoeologous genes at different times after pollination also revealed that their expression was developedally regulated. The transcription of Ta14S-2B was clearly higher during seed germination, while expressions of Ta14S-2A and Ta14S-2D were up-regulated at the beginning of seed imbibition 0-12 h), but later thereafter. The results suggested that the three Ta14 S homoeologous genes have regulatory roles in seed development and germination.