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建立超高效液相色谱法同时测定人参中的7种人参皂苷的方法。使用超高效液相分离系统,超声波萃取法,选用Waters BEH C18分析柱(2.1mm×50 mm×1.7μm),乙腈-水进行梯度洗脱,紫外检测器检测,波长为203 nm,峰面积外标法定量。7种人参皂甙在5～100μg/mL浓度范围内,其浓度和峰面积有良好的线性关系,相关系数R≥0.999 9。针对红参,白参样品加标回收率在74.1%～103.0%之间,RSD=7.41%(n=6)。本方法前处理简单,准确,重现性好,且能同时检测7种人参皂甙分析时间短,具有较大优势。此方法适用于人参及其制品中人参皂甙的测定。
To establish a method for simultaneous determination of seven kinds of ginsenosides in ginseng by ultra performance liquid chromatography. The sample was separated on a Waters BEH C18 column (2.1 mm × 50 mm × 1.7 μm) using an ultra-high performance liquid chromatography (HPLC) separation system using ultrasonic extraction. The sample was eluted with acetonitrile-water gradient and detected by UV detector at a wavelength of 203 nm. Standard method of quantitative. Seven kinds of ginsenosides in the concentration range of 5 ~ 100μg / mL, the concentration and peak area has a good linear relationship, the correlation coefficient R ≥ 0.9999. The recovery of spiked samples was between 74.1% and 103.0%, RSD was 7.41% (n = 6). The method has simple pretreatment, accurate and good reproducibility, and can simultaneously detect seven kinds of ginsenosides with short analysis time and has greater advantages. This method is suitable for the determination of ginsenosides in ginseng and its products.