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病毒围栏增殖方法由云南省林科院经过１０多年病毒复制试验证明：该方法是增殖ＣＰＶ用以防治松毛虫最经济实用的方法。其病毒投入少，产量高，风险较小。设置围栏采集６～７龄文山松毛虫幼虫，选用１×１０７ＣＰＢ／ｍＬ的病毒感染浓度，接种后１３～１５天回收，感病虫回收率可达８０％以上，单虫多角体病毒含量达２０亿以上。本文详细介绍了围栏制作，增殖场地选择，围栏管理，最适病毒感染浓度及感病虫的回收时间和采集增殖病毒幼虫的方法。
Virus fence method of proliferation by the Yunnan Academy of Forestry after 10 years of virus replication test proved that: This method is the proliferation of CPV for the prevention and control of pine caterpillars the most economical and practical method. The small amount of virus, high yield, less risk. Set the fence to collect 6- to 7-day-old Dendrolimus sinicus larvae, the choice of 1 × 107CPB / mL of the virus infection concentration, 13 to 15 days after inoculation recovery, susceptible pest recovery rate of up to 80%, single insect polyhedrosis virus content of 2 More than 0 million. This article details the fence production, selection of breeding sites, fence management, the optimal concentration of virus infection and the recovery time of the susceptible pests and methods of collecting the virus larvae.