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目的：研究α常春藤皂甙（Ｈｅｄ）和无患子皂甙Ｂ（ＳａｐＢ）对小鼠肝细胞色素Ｐ４５０的影响与保肝作用的关系．方法：给小鼠ｓｃＨｅｄ，ＳａｐＢ和Ｈｅｄ＋ＳａｐＢ（１∶１５），检测小鼠肝微粒体细胞色素Ｐ４５０的含量．结果：Ｈｅｄ２０ｍｇ·ｋｇ－１，ＳａｐＢ２０ｍｇ·ｋｇ－１和Ｈｅｄ＋ＳａｐＢ２０ｍｇ·ｋｇ－１使肝细胞色素Ｐ４５０分别降低了４０％，５５％和５０％．这种抑制作用在３ｄ后基本恢复正常．苯巴比妥ｉｐ５０ｍｇ·ｋｇ－１使小鼠肝细胞色素Ｐ４５０增加２５倍，Ｈｅｄ＋ＳａｐＢ使苯巴比妥诱导的Ｐ４５０降低了５０％，小鼠肝微粒体体外与Ｈｅｄ＋ＳａｐＢ共孵对Ｐ４５０没有影响．结论：Ｈｅｄ和ＳａｐＢ的保肝作用至少在某一方面是由于降低了肝细胞色素Ｐ４５０而产生的．
Objective: To study the effects of α-ivy (S) and saponin B (SapB) on hepatocyte cytochrome P450 in mice and the effect of protecting liver. Methods: The mouse scHed, SapB and Hed + SapB (1: 1 5), the detection of mouse liver microsomal cytochrome P 450 content. Results: Hed 20mg · kg-1, SapB 20mg · kg-1 and Hed + SapB 20mg · kg-1 decreased the cytochrome P450 by 40%, 55% and 50% respectively. This inhibitory effect returned to normal after 3d. Phenobarbital ip50mg · kg-1 increased mouse liver cytochrome P450 by 2.5 times, Hed + SapB reduced phenobarbital-induced P5050 by 50%, and mouse liver microsomes were incubated with Hed + SapB in vitro P 450 has no effect. Conclusion: The hepatoprotective effects of Hed and SapB are at least partly due to the reduction of hepatocyte cytochrome P450.