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目的分离并鉴定我国首例输入性D9基因型麻疹病毒。方法使用Vero/SLAM细胞(非洲绿猴肾细胞/淋巴信号激活因子转染的非洲绿猴肾细胞)分离病毒,使用逆转录-聚合酶链反应扩增出编码核蛋白羧基末端450个核苷酸片段,通过对扩增产物进行核苷酸序列测定和分析,构建基因亲缘性关系树,进行遗传距离分析。结果四川省D9基因型麻疹病毒分离株MVi/Sichuan.CHN/07.09/1和世界卫生组织D9基因型代表株Victoria.AUS(维多利亚?澳大利亚)/12.99在基因亲缘性关系树上同属一个分支,核苷酸同源性为96.9%;和2008年流行于泰国和荷兰的D9基因型麻疹野病毒的核苷酸和氨基酸同源性分别为99.8%～100%和99.3%～100%;和中国大陆目前所使用的麻疹疫苗株沪191相比对,其核苷酸和氨基酸同源性分别为92.3%和90.7%;和中国目前流行的麻疹病毒绝对优势本土基因型H1基因型代表株相比对,其核苷酸和氨基酸同源性分别为90.8%和92.1%。结论输入中国四川省的麻疹病毒株为D9基因型。
Objective To isolate and identify the first imported D9 genotype measles virus in China. Methods Viruses were isolated using Vero / SLAM cells (African green monkey kidney cells transfected with African green monkey kidney cell / lymphotactin activator) and 450 nucleotides encoding the carboxy terminal of the nucleoprotein were amplified using reverse transcription-polymerase chain reaction Fragment, the nucleotide sequence of the amplified product was measured and analyzed to construct gene relationship tree, genetic distance analysis. Results The genotype D9 MVi / Sichuan.CHN / 07.09 / 1 and the representative strain of Victoria D9 genotype Victoria.AUS (Victoria, Australia) /12.99 belong to the same branch of phylogenetic tree, Nucleotide homology was 96.9%. The nucleotide and amino acid homology of wild type D9 genotype wild virus in Thailand and the Netherlands in 2008 were 99.8% -100% and 99.3% -100%, respectively; The presently used measles vaccine strain Shanghai 191 compared to its nucleotide and amino acid homologies were 92.3% and 90.7%; and China’s current epidemic of measles virus absolute advantage native genotypes H1 genotype representative strains compared to The nucleotide and amino acid homologies were 90.8% and 92.1% respectively. Conclusion The measles virus strain imported into Sichuan Province of China was D9 genotype.