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目的了解2011年-2012年江西省儿童急性出血性结膜炎病原学特征。方法采用PCR和逆转录PCR(RTPCR)检测方法,对2011年-2012年江西省急性出血性结膜炎患儿结膜拭子标本同时进行肠道病毒70型、柯萨奇病毒24型变异株和腺病毒3种病毒核酸检测。结果 2011年-2012年江西省儿童流行性出血性结膜炎各月份均有发病,均为年初开始发病,且呈逐月增多,于5月-7月最高。2011年结膜拭子标本病毒核酸阳性率为77.78%。其中,腺病毒占98.10%,肠道病毒70型占1.90%;2012年结膜拭子标本中病毒核酸阳性率为70.45%,均为腺病毒。结论 2011年-2012年江西省儿童急性出血性结膜炎流行高峰为5月-7月,病原谱构成为腺病毒占病毒阳性的比率上升,肠道病毒比率则下降。
Objective To understand the etiological characteristics of acute hemorrhagic conjunctivitis in children from 2011 to 2012 in Jiangxi Province. Methods Conjunctival swab samples from children with acute hemorrhagic conjunctivitis in Jiangxi province from 2011 to 2012 were enrolled in this study. Simultaneous enterovirus 70, Coxsackievirus 24 mutants and adenocarcinomas were detected by PCR and RT-PCR (RTPCR) Virus 3 kinds of virus nucleic acid detection. Results From 2011 to 2012, epidemic hemorrhagic conjunctivitis of children in Jiangxi Province all had morbidity in each month, all of them were onset of disease at the beginning of the year, and increased month by month with the highest in May-July. In 2011, the positive rate of conjunctival swab virus was 77.78%. Among them, adenovirus accounted for 98.10% and enterovirus 70 accounted for 1.90%. In 2012, the positive rate of viral nucleic acid in conjunctival swab specimens was 70.45%, all of which were adenovirus. Conclusion The prevalence of acute hemorrhagic conjunctivitis in children from May to July in Jiangxi Province from 2011 to 2012 shows that the prevalence of adenovirus is positive for adenovirus and the rate of enterovirus is decreased.