论文部分内容阅读
目的:探讨补肾益气法中药预防家兔膝骨性关节炎的机制。方法:4月龄健康日本长耳大白兔,72只,雄性,体重为(1.8~3.5)kg,随机分为六组,空白组(A组)、模型组(B组)、中药高剂量组(C组)、中药中剂量组(D组)、中药小剂量组(E组)、盐酸氨基葡萄糖胶囊对照组(F组),每组12只。除A组外,其余均参考Hulth造模方法造兔膝骨性关节炎模型,在造模同时C、D、E、F组分别给予不同剂量补肾益气中药及盐酸氨基葡萄糖胶囊灌胃4周,以行预防治疗,造模6周后均采用耳中央动脉取血,应用ELISA法检测血清中AQP1及AQP3的含量,同时取关节软骨行大体及光镜检查。结果:C、D、E、F组与B组血清中AQP1及AQP3含量比较均有显著性差异(P<0.05),C组、A组与B组比较有显著性差异(P<0.05),补肾益气中药中剂量组与盐酸氨基葡萄糖胶囊组无明显差异(P>0.05),补肾益气中药高、中、低剂量组之间比较有著性差异(P<0.05)。组织学显示预防治疗各组动物关节软骨有不同程度的色泽变化,骨赘、骨囊肿形成,软骨关节面出现浅表性糜烂,部分标本软骨缺损深达软骨中层,出现软骨剥脱,但程度明显低于模型组。结论:补肾益气法中药可能通过降低兔血清中AQP1及AQP3含量,来达到预防骨性关节炎的发生。
Objective: To investigate the mechanism of Bushenyiqi method in preventing knee osteoarthritis in rabbits. Methods: A total of 72 healthy male Japanese white rabbits aged 4 months were randomly divided into six groups (A group), model group (B group), high dose Chinese herb group (Group C), medium dose group (group D), traditional Chinese medicine low dose group (group E) and group A (group D). In addition to group A, the rest were made with reference to Hulth modeling rabbit knee osteoarthritis model, in the modeling while C, D, E, F were given different doses of Bushen Yiqi Chinese medicine and glucosamine hydrochloride capsules orally for 4 weeks , To prevent the treatment. After 6 weeks of modeling, the blood was collected from the central auricular artery. The contents of AQP1 and AQP3 in the serum were detected by ELISA. Gross cartilage and light microscopy were also performed. Results: The levels of AQP1 and AQP3 in serum of group C, D, E, F and B were significantly different (P <0.05), but there was significant difference between group C and group A (P <0.05) There was no significant difference (P> 0.05) between the middle dose of Bushen Yiqi Chinese herbal medicine group and the glucosamine hydrochloride capsule group (P <0.05). Histology showed prevention and treatment of articular cartilage in different groups of animals had varying degrees of color change, osteophytes, bone cysts, cartilage surface superficial erosion, cartilage defects in some of the depth of cartilage defects, cartilage exfoliation, but the degree was significantly lower In the model group. Conclusion: Bushenyiqi traditional Chinese medicine may prevent osteoarthritis by reducing the content of AQP1 and AQP3 in rabbit serum.