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目的:依托蛋白质二维液相色谱技术,建立维吾尔族妇女宫颈鳞癌早期患者特异性血浆低丰度蛋白质组图谱鉴别模型。方法:收集维吾尔族妇女血浆标本共48例,临床分期为Ⅰ～Ⅱa的宫颈鳞癌早期患者26例,慢性宫颈炎患者22例。分别制备血浆低丰度蛋白质组样品并通过蛋白质二维液相色谱系统(ProteomeLah~(TM)PF-2D)分离与鉴别分析。结果:确定了宫颈炎和早期宫颈鳞癌患者的基于蛋白质等电点和疏水性特征的血浆低丰度蛋白质组图谱,筛选出9个差异表达明显的蛋白位点,在宫颈鳞癌早期患者血浆中8个低丰度蛋白组分含量明显上升,1个组分含量下降。结论:建立了宫颈癌早期患者特异性血浆蛋白质组差异图谱鉴别模型,提示二维液相色谱技术作为一种高通量技术手段在血浆蛋白质组学研究中有很大的应用潜力。
OBJECTIVE: To establish a discriminant model of low plasma protein profile of early stage patients with cervical squamous cell carcinoma in Uighur based on protein two-dimensional liquid chromatography. Methods: A total of 48 Uygur women with plasma samples were collected from 26 patients with cervical squamous cell carcinoma of early stage Ⅰ ~ Ⅱa and 22 patients with chronic cervicitis. Plasma low abundance proteome samples were separately prepared and separated and identified by ProteomeLah ™ (TM) PF-2D. Results: The plasma low-abundance proteome map based on the isoelectric point and hydrophobicity of proteins in cervicitis and early cervical squamous cell carcinoma was identified, and 9 differentially expressed protein spots were screened out. In the early stage of cervical squamous cell carcinoma The contents of eight low-abundance proteins in plasma increased significantly, and the content of one component decreased. Conclusion: The identification model of differential proteome in patients with early stage cervical cancer is established, which indicates that two-dimensional liquid chromatography has great potential as a high-throughput technique in the study of plasma proteomics.