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本文建立了测定炎痛喜康血药浓度的高效液相色谱法。酸化的血浆用乙醚5ml提取,以1-(2-吡啶偶氮)-2-萘酚为内标,提取溶剂吹干后用甲醇溶解进样。所用固定相为多孔硅胶-氰基(10μm),流动相为pH3的甲醇-0.1mol/L磷酸二氢钾(45∶55)。本法测得样品的最低检测限为2ng;最低检测浓度为50ng/ml(S/N>2),线性范围为0.2～10μg/ml。方法的平均相对回收率为99.5%,日内与日间精密度的变异系数均小于5%。
In this paper, the establishment of a determination of inflammatory pain Xikang blood concentration of high performance liquid chromatography. The acidified plasma was extracted with 5 ml of diethyl ether and 1- (2-pyridylazo) -2-naphthol as internal standard. The extracted solvent was dried and dissolved in methanol for injection. The stationary phase used was porous silica-cyano (10 μm), methanol-0.1 mol / L potassium dihydrogen phosphate (45:55) with a mobile phase of pH 3. The minimum detectable limit of detection of this method is 2ng. The lowest detection limit is 50ng / ml (S / N> 2) and the linear range is 0.2-10μg / ml. The average relative recovery of the method was 99.5%, and the coefficient of variation of intra-day and inter-day precision was less than 5%.