论文部分内容阅读
目的:利用ITS2序列鉴别木槿及其常见混伪品基原植物。方法:提取木槿及其混伪品共9个样本DNA、PCR扩增和测序,得到ITS2序列,结合GenBank下载的12条ITS2序列,计算种内种间Kimura 2-parameter(K2P)遗传距离并构建NJ系统聚类树。结果:木槿与其混伪品之间的K2P遗传距离为0.236~0.301,远大于木槿种内K2P遗传距离(0.009~0.056);木槿种内序列变异位点为2~9个,远少于与混伪品的变异位点(45~52个);NJ系统聚类树显示木槿种内不同个体聚在一起,与其混伪品明显分开。结论:ITS2条形码可准确鉴别木槿及其混伪品基原植物,为木槿及其混伪品鉴定提供了分子依据。
OBJECTIVE: To identify Hibiscus hibiscus using its ITS2 sequence and its common plant species. Methods: Nine samples of Hibiscus mariqueter and its adulterant were extracted and sequenced. The ITS2 sequence was obtained by PCR. The 12 ITS2 sequences downloaded from GenBank were used to calculate the genetic distance of inter-species Kimura 2-parameter (K2P) NJ system clustering tree. Results: The K2P genetic distance between H. hibiscus and its adulterants ranged from 0.236 to 0.301, which was much larger than the K2P genetic distance (0.009-0.056) in Hibiscus species. The variation range of Hibiscus species varied from 2 to 9, (45 ~ 52). NJ phylogenetic tree showed that different individuals in Hibiscus species were clustered together, obviously separated from their adulterants. Conclusion: ITS2 barcode can accurately identify Hibiscus marijuana and its adulterants base plants, providing a molecular basis for the identification of hibiscus and its adulterants.