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本文以螯虾细小神经轴突为标本,成功地应用双玻璃微电极进行细胞内刺激与电活动的记录。观察了细小神经轴突膜电生理和物理特性参数。钾离子通道特异性阻滞剂4—氨基吡啶,使Weiss式中常数b值显著减少;a值无明显变化;膜静息电位去极化;阈电位水平上移;动作电位幅值、锋电位时程、膜电阻和膜时间常数均有显著意义的增加。结果提示:4—氨基吡啶可提高膜的应激性,对神经膜兴奋性无明显影响;生物膜应激性及膜电阻的变化与钾通道的活动状态有密切关系。
In this paper, the crayfish neuromuscular axon as a specimen, the successful application of double glass microelectrode for intracellular stimulation and electrical activity recorded. The electrophysiological and physical properties of small axons were observed. 4-aminopyridine, a potassium channel blocker, significantly reduced the b value in the Weiss formula; there was no significant change in a value; depolarization of membrane resting potential; the threshold potential level was shifted upward; the amplitude of action potential, Time course, membrane resistance and membrane time constant were significantly increased. The results suggest that 4-aminopyridine can improve the membrane stress and have no significant effect on the excitability of the neural membrane. The changes of biofilm stress and membrane resistance are closely related to the activity of potassium channels.