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目的研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14C同位素标记的特异底物“尿苷二磷酸-(14C)-葡萄糖”示踪,研究捷安肽素对真菌(1,3)-β-D-葡聚糖合成酶活性反应的影响。结果研究神经生长因子(NGF)在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学ABC法染色,研究35天人胚的发育情况。结果在人胚神经管的室管带中,神经元的细胞质和细胞核NGF免疫反应阳性;在中间带,一部分神经元的细胞核NGF免疫反应阳性,另外一部分神经元的细胞核NGF免疫反应阴性,而其突起NGF免疫反应阳性;在边缘带NGF的表达与中间带相似。在神经管的头侧NGF阳性反应较强,神经管的尾侧NGF阳性反应较弱。结论NGF在人胚神经管免疫反应阳性,表明NGF可能是诱导神经管分化发育的重要信号分子,提示NGF可能在人胚神经管的发育中具有十分重要的作用。
Objective To study the antifungal mechanism of guanacin. Methods Morphological methods and isotope labeling method. Morphological changes of the tested fungi after treatment with Galectin were observed by microscopic observation. Further, 14c-labeled specific substrate “uridine diphosphate- (14C) -glucose” was used to study the effect of gentamycin on fungal (1,3) -β-D-glucan synthase activity Impact. Results The localization of nerve growth factor (NGF) in early human embryonic neural tube development was studied. Methods Immunocytochemical ABC staining was used to study the development of human embryo on day 35. Results NGF immunoreactivity was positive in the cytoplasm and nucleus of neurons in the ependymal zone of human embryonic neural tube. In the middle zone, NGF immunoreactivity was positive in nuclei of some neurons and negative in nuclei of NGF in other neurons. Prominent NGF immunoreactivity was positive; NGF expression was similar in the marginal zone to the midgut. NGF positive reaction in the head of the neural tube is strong, and the NGF positive reaction in the tail of the neural tube is weak. Conclusions NGF is positive in the neural tube of human embryo, indicating that NGF may be an important signaling molecule that induces the development of neural tube. It suggests that NGF may play an important role in the development of human neural tube.