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调节性T细胞和调节性NK细胞都可能在维持妊娠方面发挥关键作用。研究显示,同种异基因妊娠非肥胖型糖尿病(NOD)小鼠胚胎丢失率显著增高,而注射抗唾液酸神经节苷脂清除其残存的NK细胞,可使胚胎丢失率进一步增高。相反,过继性输注来源于正常小鼠的CD49b+CD25+NK细胞可显著降低胚胎丢失率。检测了CXCL12的特异性受体CXCR4在NK细胞表面的表达模式,并采用体外和体内迁移实验研究了NK细胞的迁移规律。由于以往研究证实小鼠胎盘滋养层细胞表达CXCL12,研究结果提示:孕期动员子宫外表达CXCR4的CD49b+CD25+NK细胞向妊娠子宫内迁移,可能对于调节母胎免疫耐受具有重要意义。
Both regulatory T cells and regulatory NK cells may play a key role in the maintenance of pregnancy. Studies have shown that allogeneic pregnancy non-obese diabetic (NOD) mice embryos loss rate was significantly increased, while the injection of anti-sialic acid ganglioside removal of its remaining NK cells, embryos can further increase the loss rate. In contrast, adoptive infusion of CD49b + CD25 + NK cells derived from normal mice resulted in a significant reduction in embryo loss. The expression of CXCR4, a specific receptor of CXCL12, on the surface of NK cells was examined. The migration of NK cells was studied by in vitro and in vivo migration assays. As previous studies confirmed CXCL12 expression in mouse placental trophoblast cells, the results suggest that mobilization of CD49b + CD25 + NK cells expressing CXCR4 in uterus during pregnancy may play an important role in regulating the immune tolerance of maternal fetus.