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目的:观察血必净注射液对宫内感染致早产脑损伤仔鼠脑组织肿瘤坏死因子(TNF-α)和胶质纤维酸性蛋白(GFAP)表达的影响。方法:①12只孕第16天脂多糖(LPS)组大鼠予LPS 450μg.kg-1,ip,连续2 d,8只生理盐水组孕鼠等量生理盐水ip。孕22 d前分娩的仔鼠为早产仔鼠。随机选取生理盐水组足月产仔鼠8只作为空白对照组和LPS组早产仔鼠24只。LPS组仔鼠随机分为3组,每组8只,分别为血必净高、低剂量(4,2 g.kg-1)组,模型组。7日龄时开始分别给予血必净注射液或生理盐水,ip,共14 d。②21日龄时对4组大鼠进行神经行为学检测(悬吊试验),并取脑组织,用免疫组化方法测定GFAP,TNF-α水平。结果:与模型组比较,血必净注射液高、低剂量组干预治疗后脑损伤仔鼠行为学方面得到改善(P<0.05);与模型组比较,血必净注射液高、低剂量组干预治疗后,早产脑损伤仔鼠脑组织TNF-α阳性表达减少而GFAP阳性表达增加(P<0.05)。结论:化瘀解毒中药血必净注射液可改善宫内感染所致早产脑损伤,其作用机制可能与降低脑组织TNF-α表达和使GFAP表达升高有关。
Objective: To observe the effect of Xuebijing injection on the expression of tumor necrosis factor (TNF-α) and glial fibrillary acidic protein (GFAP) in neonatal rats with premature brain injury induced by intrauterine infection. Methods: ①12 pregnant rats on the 16th day of lipopolysaccharide (LPS) were given LPS 450μg.kg-1, ip for 2 days, and the rats in the 8 normal saline groups were given the same amount of saline ip. Premature delivery of offspring of offspring born 22 days before pregnancy. Totally 8 full-term offspring rats in saline group were randomly selected as control group and 24 premature pups in LPS group. LPS group were randomly divided into 3 groups, 8 in each group, which were high and low dose Xuebijing (4,2 g.kg-1) group, model group. 7 days of age were given Xuebijing injection or saline, ip, a total of 14 d. ② The neurobehavioral test (suspension test) was performed on the 4-day-old rats at 21 days of age and the brain tissue was taken out. The levels of GFAP and TNF-α were determined by immunohistochemistry. Results: Compared with the model group, the behavior of offspring of Xuebijing injection group after high and low dose intervention was improved (P <0.05). Compared with the model group, the intervention of Xuebijing Injection in high and low dose groups After treatment, the positive expression of TNF-α and the positive expression of GFAP in brain tissue of premature born rats were decreased (P <0.05). Conclusion: Huayu Jie injection of Huayu detoxification can improve brain injury induced by intrauterine infection, and its mechanism may be related to the decrease of TNF-α expression and the increase of GFAP expression in brain tissue.