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目的研究菌核净内分泌干扰作用(雌激素和抗雄激素活性作用)。方法通过不同实验终点探讨菌核净雌激素样作用:(1)每隔5天腹腔注射给予鲫鱼不同浓度菌核净(0、50、100和200mg/kg)诱导卵黄蛋白原(VTG)的产生,2周后收集雄性鲫鱼血浆和肝脏进行VTG检测;(2)人乳腺癌细胞(MCF-7细胞)中加入不同浓度的菌核净(10-10～10-4mol/L),作用3天后观察菌核净诱导细胞增殖情况;(3)雌性小鼠去势后给予不同浓度菌核净(0、125、250和500mg/kg),计算子宫脏器系数。Hershberger试验研究菌核净抗雄激素样作用:雄性大鼠去势后给予不同剂量的菌核净(0、30、60和120mg/kg),同时皮下注射雄性激素丙酮酸睾酮(TP),计算雄激素依赖组织的脏器系数。结果100mg/kg和200mg/kg菌核净能诱导雄性鲫鱼血VTG产生,200mg/kg菌核净能刺激雄性鲫鱼肝脏产生VTG,与阴性对照组相比,VTG含量差异均具有显著性(P<0.05);各剂量组菌核净对雄性鲫鱼血钙离子、血和肝总蛋白含量均无影响,但阳性对照E2能增高血钙离子水平;10-8、10-7和10-6mol/L菌核净能诱导MCF-7细胞增殖,与对照组相比,差异具有显著性(P<0.05);菌核净3个剂量组均能增加去势小鼠子宫脏器系数,其中250mg/kg组与阴性对照组比较差异具有显著性(P<0.05);60mg/kg和120mg/kg菌核净均能降低前列腺加精囊腺、肛提肌、球海绵体肌的脏器系数,与模型对照组比较,差异具有显著性(P<0.05)。结论菌核净具有雌激素和抗雄激素双重内分泌干扰作用。
Objective To study the role of sclerotin in endocrine disruption (estrogen and anti-androgen activity). Methods The net estrogen-like activity of sclerotia was investigated by different experimental end points: (1) The net (0, 50, 100 and 200 mg / kg) sclerotis of Carassius auratus was induced intraperitoneally every 5 days to induce vitellogenin (VTG) (2) Human breast cancer cells (MCF-7 cells) were treated with different concentrations of sclerotium (10-10 ~ 10-4 mol / L) for 3 days (3) Female mice were scopulated with different concentrations of sclerotium (0,125,250 and 500 mg / kg) to calculate the coefficient of uterine organ. Hershberger Experimental Study of Sclerotinic Anti-Androgen-like Effect: Male rats were scopulated with different doses of sclerotinum (0, 30, 60 and 120 mg / kg) and testosterone pyruvate (TP) was injected subcutaneously Organ coefficient of androgen-dependent tissues. Results The net energy of 100 mg / kg and 200 mg / kg sclerotium induced the blood VTG production in male crucian carp, and the net caloric value of 200 mg / kg sclera stimulated the production of VTG in the liver of the crucian carp. Compared with the negative control group, the VTG content was significantly different (P < 0.05). The sclerotium of each dose group had no effect on the content of calcium, blood and liver total protein in male Carassius auratus, but the positive control E2 increased the level of serum calcium ion. The levels of 10-8, 10-7 and 10-6 mol / L Sclerotium induced net proliferation of MCF-7 cells, and the difference was significant compared with the control group (P <0.05). The three doses of sclerotin increased the uterine organ coefficient of ovariectomized mice, 250mg / kg (P <0.05). The sclerotin of prostatic and seminal vesicle, levator ani muscle and ball cavernosum of 60mg / kg and 120mg / kg sclerenchyma could both reduce the organ coefficient of model group Group, the difference was significant (P <0.05). Conclusions Sclerotinum has a dual endocrine disrupting effect of estrogen and anti-androgen.