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目的:研究缺血时豚鼠左心室心内、外膜细胞及M细胞缓慢激活延迟整流钾通道电流（Iks）的变化特性。方法:利用全细胞膜片钳技术观察豚鼠左心室心内、外膜细胞及M细胞Iks变化,利用不同成分浴槽液模拟细胞正常及缺血环境,观察Iks尾电流锋值的变化情况.结果:缺血时,三层细胞Iks均小于正常状态;指令电压小于0 mV时,三层细胞Iks同步减小,减少率无显著性差异,P>0.05。指令电压≥0mV时,M细胞Iks减少程度明显高于心内、外膜细胞,P<0.05;而心内、外膜细胞减少率无显著性差异,P>0.05。结论:缺血时左心室心内、外膜细胞及M细胞Iks减弱,而M细胞Iks减弱更为明显,这可能会增加心室壁电活动不均一性,诱发心律失常。
Objective: To study the characteristics of delayed activation of delayed rectifier potassium channel currents (Iks) in left and right ventricular myocardium and M cells of ischemic guinea pigs. Methods: Whole-cell patch-clamp technique was used to observe the changes of Iks in the cardiac and epicardial cells and M cells in guinea pig ventricular myocytes, and the normal and ischemic environments of the cells were simulated by bath compositions of different components to observe the changes of the Iks tail current front.Results: Blood, Iks were lower than the normal three-tier cells; command voltage is less than 0 mV, three layers of cells Iks decreased simultaneously, no significant difference in the rate of reduction, P> 0.05. When the command voltage was greater than or equal to 0 mV, the decrease of Iks in M cells was significantly higher than that in the heart and adventitia cells (P <0.05). However, there was no significant difference in the decrease rate of intracardiac and adventitial cells (P> 0.05). CONCLUSION: Iks in I / M and M cells in the left ventricle are weakened while the Iks in M cells are weakened more obviously during ischemia, which may increase the inhomogeneity of ventricular wall electrical activity and induce arrhythmia.