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大豆水溶性蛋白是大豆籽粒蛋白质的主要组成部分,其含量是评价大豆品质及衡量其价值的重要指标。以高水溶性蛋白含量(38.70±1.32)%的冀豆12和正常水溶性蛋白含量(32.40±1.08)%的黑豆(ZDD03651)为亲本构建的包含188个家系的F8、F9重组自交系群体为材料,利用双尾法在3个不同选择压力下对籽粒水溶性蛋白含量进行QTL定位研究。结果表明,2年中重组自交家系群体表型均呈现正态性超双亲分离,在30%,20%,10%这3个不同选择压力下共检测到大豆籽粒水溶性蛋白含量相关的QTL 23个,分布在A1、A2、C2、D1b、D2、E、F、H、I、K、L、M和O等13个连锁群中,其中13个QTL增效基因来自冀豆12。2年中在10%的选择压力下同时检测到2个QTL,分别为qSPC-6-1和qSPC-8-1。其中qSPC-6-1的连锁标记为Sat_062,2年中贡献率分别为9.78%和9.66%,增效基因来自冀豆12;qSPC-8-1的连锁标记为Satt177,2年中贡献率分别为9.78%和7.98%,增效基因来自于黑豆。该结果为分子标记辅助育种及分子克隆提供理论依据。
Soybean water-soluble protein is the main component of soybean protein, its content is an important index to evaluate the quality of soybean and measure its value. A total of 188 F8 and F9 recombinant inbred lines (PBMCs) of Jidou 12 with high water-soluble protein content (38.70 ± 1.32%) and black bean (ZDD03651) with normal water-soluble protein content of 32.40 ± 1.08% As the material, the two-tail method was used to study the QTL mapping of grain water-soluble protein under three different selection pressures. The results showed that the phenotypes of the two groups showed normal polymorphic parents, and the QTLs associated with water soluble protein content in soybean grains were detected under three different selection pressures of 30%, 20% and 10% 23 of them were distributed in 13 linkage groups of A1, A2, C2, D1b, D2, E, F, H, I, K, L, M and O, among which 13 QTLs were from Jidou 12.2 Two QTLs were simultaneously detected at 10% selection pressure during the year, qSPC-6-1 and qSPC-8-1, respectively. Among them, qSPC-6-1 was labeled as Sat_062, with a pay-off rate of 9.78% and 9.66% for 2 years and a synergistic gene from Jidou 12. The linkage of qSPC-8-1 was Satt177, 9.78% and 7.98%, respectively. The synergistic genes were from black beans. The results provide a theoretical basis for molecular marker-assisted breeding and molecular cloning.