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CD134,a member of the tumor necrosis factor receptor superfamily,plays a crucial role in T cell survival.In thisstudy,peripheral blood mononuclear cells(PBMCs)stimulated with phytohemagglutinin(PHA,50 μg/ml)weretreated by CD134 mAb(1 μg/ml,5 μg/ml,10 μg/ml)for 6 h,12 h,24 h and 48 h.The level of perforin mRNA wasmeasured by reverse transcription-polymerase chain reaction(RT-PCR)technique.Our data showed that theexpression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in rangeof 1 μg/ml to 5 μg/ml and dropped down to its minimum on 24 h (p < 0.05).The level of perforin mRNA reached aplateau when the concentration of CD134 mAb exceeded 5 μg/ml.In conclusion,CD134 mAb can inhibit perforinexpression,which may enhance the ability of T cells for survival.Cellular & Molecular Immunology.2005;2(6):467-471.
CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 μg / ml) weretreated by CD134 mAb ml, 5 μg / ml, 10 μg / ml for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA wasmeasured by reverse transcription-polymerase chain reaction (RT- of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in a range of 1 μg / ml to 5 μg / ml and dropped down to its minimum on 24 h (p <0.05). The level of perforin mRNA reached aplateau when the concentration of CD134 mAb exceeded 5 μg / ml. In conclusion, CD134 mAb can inhibit perforinexpression, which may enhance the ability of T cells for survival. Cellular & Molecular Immunology. 2005; 2 (6): 467-471.