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目的 :建立甘草栓中甘草酸、甘草次酸的含量测定新方法 ,为中药甘草制剂的质量控制提供新的检测方法。方法 :采用高效毛细管电泳法同时测定主要成分。电解缓冲液由 2 5mmol/L硼砂溶液和 10 0mmol/L十二烷基磺酸钠 (SDS)溶液组成。熔融石英毛细管 75 μm× 6 0cm ,在 2 0kV电压下运行 ,进样时间 10s,检测波长为 2 5 4nm ,温度 2 5℃。 结果 :上述条件下 ,甘草酸、甘草次酸得到较好分离 ,加样回收率分别为 99.2 %和 99.7%,RSD分别 2 .3 %和 2 .6 %。天间和天内精密度RSD在 1.0 0 %- 3 .0 0 %(n =3)。线性浓度范围在 2 5 - 30 0 μg/ml。 结论 :该方法简便、快捷、准确 ,具良好的精密度和回收率 ,可作为中药甘草制剂的质量控制方法。
Objective : To establish a new method for the determination of glycyrrhizic acid and glycyrrhetinic acid in liquorice suppositories, and to provide a new detection method for quality control of traditional Chinese medicines. Methods: Simultaneous determination of major components by high performance capillary electrophoresis. The electrolysis buffer consisted of 25 mmol/L borax solution and 100 mmol/L sodium dodecyl sulfate (SDS) solution. Fused silica capillary 75 μm × 60 cm, operating at 20 kV, injection time 10 s, detection wavelength 254 nm, temperature 25°C. Results: Under the above conditions, glycyrrhizic acid and glycyrrhetinic acid were well separated. The recoveries of the samples were 99.2% and 99.7%, respectively, and the RSDs were 2.3% and 2.6%, respectively. Day and day precision RSDs were 1.00%-3.0% (n = 3). Linear concentrations ranged from 25 to 30 μg/ml. Conclusion: This method is simple, rapid, accurate, and has good precision and recovery rate. It can be used as a quality control method for traditional Chinese medicine licorice preparations.