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采集湖北省长阳县火烧坪乡根肿病重发区的病土和病根,通过病原菌形态和PCR鉴定,确定是芸薹根肿菌,然后利用欧洲ECD鉴别系统确定生理小种为ECD17/31/13,此病原菌致病力极强。采用田间苗期人工接种鉴定,与田间成株期自然诱发鉴定相结合,对88份甘蓝种质进行抗性评价和筛选,结果表明:苗期获得1份高抗,7份抗病,17份耐病材料;成株期获得4份高抗,4份抗病,15份耐病材料。2个时期88份材料群体抗性鉴定级别基本一致,93.18%材料成株期病指比苗期高。CR21在2个时期均为高抗,抗性最强,表现稳定;CR55在苗期发病最严重,病指达到76.19,成株期为74.10;CR54在成株期发病最严重,病指达到81.54,苗期为75.97,2个时期发病率均达到100%。根肿病菌的鉴定和致病力的确定,及甘蓝种质抗性评价为抗病品种选育和抗病机理的研究奠定基础。
Pathogenic bacteria and diseased roots were collected from the recurrence area of the root swollen disease in Huozhaoping Township, Changyang County, Hubei Province. The pathogen and diseased root were identified by morphological and PCR identification of pathogenic bacteria, then identified by the European ECD differential system as ECD17 / 31 / 13, the pathogens extremely strong. Eighty-eight cabbage germplasms were evaluated for resistance and screened by artificial inoculation identification in field seedling stage and naturally induced identification in the adult plant stage. The results showed that: one high resistance, seven resistance and 17 resistance Disease-resistant materials; 4 high resistance, 4 disease-resistant, 15 disease-resistant materials were obtained at the adult stage. The resistance identification of 88 material groups in two periods was basically the same, and 93.18% of the materials in the adult plantlets were higher than the seedling stage. CR21 was highly resistant in 2 periods, with the strongest resistance and stable performance. CR55 was most severely morbid at seedling stage with the disease index of 76.19 and the adult plant age of 74.10. The incidence of CR54 was the highest at the adult stage with 81.54 , The seedling stage was 75.97, and the incidence rate reached 100% in two stages. Identification of clubroot bacteria and determination of pathogenicity and evaluation of cabbage germplasm lay the foundation for the study of breeding of resistant varieties and disease resistance mechanism.