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[目的]探讨异叶天南星氯仿萃取物对肝癌HepG-2细胞的凋亡作用.[方法]采用MTT法测定不同质量浓度的异叶天南星氯仿萃取物对肝癌HepG-2细胞生存抑制率的影响,并利用HE染色法观察各组肝癌HepG-2细胞形态学的变化.[结果]MTT检测结果显示,异叶天南星氯仿萃取物可明显抑制肝癌HepG-2细胞的增殖(P<0.05,P<0.01),且抑制作用随给药时间的延长及药物质量浓度的增加而明显增强;当异叶天南星氯仿萃取物质量浓度为100mg/L,作用时间为48h时,对肝癌HepG-2细胞的抑制率为54.33%,接近IC50.HE染色结果显示,经异叶天南星氯仿萃取物处理的HepG-2细胞表现出细胞间距增大,细胞核固缩、深染,细胞浓缩、碎裂、形成凋亡小体等一系列凋亡形态,且凋亡程度随药物质量浓度的增加及给药时间的延长而增强.[结论]异叶天南星氯仿萃取物可诱导肝癌HepG-2细胞凋亡.
[Objective] To explore the apoptosis of HepG-2 cells induced by chloroform extract of Ampelopsis grossedentata [Method] MTT assay was used to determine the effect of different concentrations of extract of Lonicera chinensis on survival inhibition rate of HepG-2 cells. The morphological changes of HepG-2 cells were observed by HE staining. [Results] The results of MTT assay showed that the extract of Nile tilapiae could significantly inhibit the proliferation of HepG-2 cells (P <0.05, P <0.01) ), And the inhibitory effect increased with the prolongation of the administration time and the increase of the drug mass concentration. When the concentration of chloroform extract of Anemarrhena asiatica was 100 mg / L and the action time was 48 h, the inhibitory rate of HepG-2 cells was Was 54.33%, close to the IC50.HE staining results showed that HepG-2 cells treated with chloroform extract of Anemarrhena asiatis showed an increase in intercellular distance, nuclear condensation, deep staining, cell concentration, fragmentation, the formation of apoptotic bodies And a series of apoptotic morphology, and the degree of apoptosis increased with the increase of drug concentration and prolongation of administration time. [Conclusion] Extract of Anemarrhena asiatica can induce HepG-2 cell apoptosis.